Two-Dimensional and Three-Dimensional Single Particle Tracking of Upconverting Nanoparticles in Living Cells
| Autor: | Yeongchang Goh, Kang Taek Lee, Yo-Han Song, Kyujin Shin |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
intracellular transport
Fluorescence-lifetime imaging microscopy three-dimensional imaging Materials science Fluorescent Antibody Technique Nanotechnology 02 engineering and technology Review 010402 general chemistry Tracking (particle physics) 01 natural sciences Catalysis Nanomaterials law.invention Inorganic Chemistry lcsh:Chemistry Drug Delivery Systems Imaging Three-Dimensional law Ultraviolet light Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy Total internal reflection Organic Chemistry Biological Transport General Medicine 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences Computer Science Applications Molecular Imaging Lens (optics) upconversion nanoparticles lcsh:Biology (General) lcsh:QD1-999 Microscopy Fluorescence Cell Tracking Particle Nanoparticles single-particle tracking 0210 nano-technology |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 20, Iss 6, p 1424 (2019) |
| ISSN: | 1422-0067 |
| Popis: | Lanthanide-doped upconversion nanoparticles (UCNPs) are inorganic nanomaterials in which the lanthanide cations embedded in the host matrix can convert incident near-infrared light to visible or ultraviolet light. These particles are often used for long-term and real-time imaging because they are extremely stable even when subjected to continuous irradiation for a long time. It is now possible to image their movement at the single particle level with a scale of a few nanometers and track their trajectories as a function of time with a scale of a few microseconds. Such UCNP-based single-particle tracking (SPT) technology provides information about the intracellular structures and dynamics in living cells. Thus far, most imaging techniques have been built on fluorescence microscopic techniques (epifluorescence, total internal reflection, etc.). However, two-dimensional (2D) images obtained using these techniques are limited in only being able to visualize those on the focal planes of the objective lens. On the contrary, if three-dimensional (3D) structures and dynamics are known, deeper insights into the biology of the thick cells and tissues can be obtained. In this review, we introduce the status of the fluorescence imaging techniques, discuss the mathematical description of SPT, and outline the past few studies using UCNPs as imaging probes or biologically functionalized carriers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |