Remodeling of photosynthetic electron transport in Synechocystis sp. PCC 6803 for future hydrogen production from water
| Autor: | Nina Dyczmons-Nowaczyk, Daniela Kannchen, Jure Zabret, Sascha Rexroth, Pia Löbbert, Marc M. Nowaczyk, Matthias Rögner, Anna Frank, Regina Oworah-Nkruma, Katrin Wiegand |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Models Molecular Protein Conformation Mutant Biophysics Photosynthesis 01 natural sciences Biochemistry Protein–protein interaction Electron Transport 03 medical and health sciences Ferredoxin 030304 developmental biology 0303 health sciences biology Base Sequence Chemistry Synechocystis Water Cell Biology biology.organism_classification Electron transport chain In vitro Metabolic pathway Mutation bacteria Oxidoreductases 010606 plant biology & botany Hydrogen |
| Zdroj: | Biochimica et biophysica acta. Bioenergetics. 1861(8) |
| ISSN: | 1879-2650 |
| Popis: | Photosynthetic microorganisms such as the cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis) can be exploited for the light-driven synthesis of valuable compounds. Thermodynamically, it is most beneficial to branch-off photosynthetic electrons at ferredoxin (Fd), which provides electrons for a variety of fundamental metabolic pathways in the cell, with the ferredoxin-NADP+ Oxido-Reductase (FNR, PetH) being the main target. In order to re-direct electrons from Fd to another consumer, the high electron transport rate between Fd and FNR has to be reduced. Based on our previous in vitro experiments, corresponding FNR-mutants at position FNR_K190 (Wiegand, K., et al.: “Rational redesign of the ferredoxin-NADP-oxido-reductase/ferredoxin-interaction for photosynthesis-dependent H2-production”. Biochim Biophys Acta, 2018) have been generated in Synechocystis cells to study their impact on the cellular metabolism and their potential for a future hydrogen-producing design cell. Out of two promising candidates, mutation FNR_K190D proved to be lethal due to oxidative stress, while FNR_K190A was successfully generated and characterized: The light induced NADPH formation is clearly impaired in this mutant and it shows also major metabolic adaptations like a higher glucose metabolism as evidenced by quantitative mass spectrometric analysis. These results indicate a high potential for the future use of photosynthetic electrons in engineered design cells – for instance for hydrogen production. They also show substantial differences of interacting proteins in an in vitro environment vs. physiological conditions in whole cells. |
| Databáze: | OpenAIRE |
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