Bone marrow stromal proteoglycans regulate megakaryocytic differentiation of human progenitor cells

Autor: Tanja Netelenbos, Floortje L. Kessler, Peter C. Huijgens, Jeroen Janssen, Angelika M. Dräger, Jakob Van Den Born, Sonja Zweegman, Adriana M.C. Mus
Přispěvatelé: Hematology, Hematology laboratory
Jazyk: angličtina
Rok vydání: 2004
Předmět:
Lymphoma
Proteoglycans/pharmacology
Cellular differentiation
Antigens
CD34
Heparitin Sulfate/metabolism
Interleukin-8/pharmacology
Stromal Cells/cytology
Conditioned
Bone Marrow
Cell Adhesion/drug effects
Chemokine CCL4
Cells
Cultured
Chemokine CCL3
Cultured
Leukemia
biology
Lymphoma
Non-Hodgkin
Leukemia
Myeloid/metabolism
Cell Differentiation
Blood Proteins
Macrophage Inflammatory Proteins
CD34/metabolism
Cell biology
medicine.anatomical_structure
Biochemistry
Leukemia
Myeloid
Acute Disease
Major basic protein
Lymphoma
Non-Hodgkin/metabolism
Proteoglycans
Megakaryocytes
Stromal cell
Cells
Eosinophil Major Basic Protein
Stroma
Cell Adhesion
medicine
Macrophage Inflammatory Proteins/pharmacology
Humans
Antigens
Progenitor cell
Megakaryocytopoiesis
Antigens
CD34/metabolism
Hematopoietic Stem Cells/cytology
Interleukin-8
Megakaryocytes/cytology
Myeloid/metabolism
Cell Biology
Hematopoietic Stem Cells
Culture Media
carbohydrates (lipids)
Bone Marrow/pathology
Proteoglycan
Blood Proteins/pharmacology
Culture Media
Conditioned
Non-Hodgkin/metabolism
biology.protein
Heparitin Sulfate
Bone marrow
Stromal Cells
Zdroj: Experimental Cell Research, 299(2), 383-392. HANLEY & BELFUS-ELSEVIER INC
Zweegman, S, Van Den Born, J, Mus, A M C, Kessler, F L, Janssen, J J W M, Netelenbos, T, Huijgens, P C & Dräger, A M 2004, ' Bone marrow stromal proteoglycans regulate megakaryocytic differentiation of human progenitor cells ', Experimental Cell Research, vol. 299, no. 2, pp. 383-392 . https://doi.org/10.1016/j.yexcr.2004.06.018
Experimental Cell Research, 299(2), 383-392. Academic Press Inc.
ISSN: 0014-4827
DOI: 10.1016/j.yexcr.2004.06.018
Popis: Adherence of hematopoietic progenitor cells (HPCs) to stroma is an important regulatory step in megakaryocytic differentiation. However, the mechanisms through which megakaryocytic progenitors are inhibited by stroma are poorly understood. We examined the role of sulfated glycoconjugates, such as proteoglycans (PGs), on human bone marrow stroma (hBMS). To this end, PG structure was altered by desulfation or enzymatic cleavage. PGs participated in adhesion of human HPC, as desulfation resulted in about 50% decline in adhesion to hBMS. Heparan sulfate proteoglycans (HSPGs) were found to be responsible by showing about 25% decline in adhesion after pre-incubation of HPC with heparin and about 15% decline in adhesion after enzymatic removal of HSPGs from hBMS. Furthermore, PGs were involved in binding cytokines. Both desulfation and enzymatic removal of stromal HSPGs increased release of megakaryocytopoiesis- inhibiting cytokines, that is, interleukin-8 (IL-8, 1.9-fold increase) and macrophage inflammatory protein-1α (MIP-1α, 1.4-fold increase). The megakaryocytic output of HPC grown in conditioned medium of desulfated stroma was decreased to 50% of the megakaryocytic output in CM of sulfated stroma.From these studies, it can be concluded that PGs in bone marrow, in particular HSPGs, are involved in binding HPC and megakaryocytopoiesis-inhibiting cytokines. Bone marrow stromal PGs thus reduce differentiation of HPC toward megakaryocytes. © 2004 Elsevier Inc. All rights reserved.
Databáze: OpenAIRE
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