Wide mapping of a T-DNA insertion site in oilseed rape using bulk segregant analysis and comparative mapping

Autor: Michel Renard, N. Foisset, Frédérique Eber, Anne-Marie Chèvre, Pierre Barret, Régine Delourme, P. Dupuis, A. Barancer
Přispěvatelé: ProdInra, Migration, UMR 0118 UMR INRA / ENSAR : Génétique et amélioration des plantes, Institut National de la Recherche Agronomique (INRA)-Génétique et amélioration des plantes (G.A.P.)-UMR INRA / ENSAR : Génétique et amélioration des plantes (RENN UMR GENET AMELIOR PLANTES)
Jazyk: angličtina
Rok vydání: 1997
Předmět:
Zdroj: Scopus-Elsevier
Plant Breeding
Plant Breeding, Wiley, 1997, 116, pp.553-560
ISSN: 0179-9541
1439-0523
Popis: Doubled haploid oilseed rape lines segregating for a transgene inducing herbicide resistance (bar gene) were investigated for the wide mapping of the T-DNA insertion site. Bulk segregant analysis using presence/ absence and intensity polymorphisms between the bulks, as well as comparative mapping with a linkage group deriving from another cross, led to the identification of 11 random amplified polymorphic DNA (RAPD) markers tightly or loosely linked to the bar gene. Ten RAPD loci out of 11 were located on the same side of the bar locus, strongly suggesting that the T-DNA integrated in a telomeric or subtelomeric position. The eleventh RAPD marker exhibited a strong segregation distortion, which could be the result of a heteroduplex formation. Comparison of the linkage groups obtained from the two crosses showed different recombination rates between markers, possibly reflecting differences in parental genetic backgrounds. Consequences and potential applications in transgene dispersal safety assessment studies are discussed.
Databáze: OpenAIRE
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