Protective immunity to tuberculosis with Ag85B-ESAT-6 in a synthetic cationic adjuvant system IC31
| Autor: | Graham J. Hatch, Ida Rosenkrands, Peter Andersen, Ann Williams, Constantia Kritsch, Alexander von Gabain, Karen Lingnau, Else Marie Agger, Claire Swetman Andersen, Karen Smith Korsholm, Anja Weinreich Olsen |
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| Rok vydání: | 2006 |
| Předmět: |
Tuberculosis
medicine.medical_treatment Guinea Pigs Guinea pig Mice Immune system Adjuvants Immunologic Bacterial Proteins medicine Animals Secretion Receptor Tuberculosis Vaccines Antigens Bacterial Mice Inbred BALB C General Veterinary General Immunology and Microbiology business.industry Public Health Environmental and Occupational Health TLR9 medicine.disease Recombinant Proteins Mice Inbred C57BL Infectious Diseases ESAT-6 Immunology Molecular Medicine Female business Adjuvant |
| Zdroj: | Vaccine. 24(26) |
| ISSN: | 0264-410X |
| Popis: | In this study, we evaluated the potential of a novel synthetic adjuvant designated IC31 for the ability to augment the immune response and protective efficacy of the well-known mycobacterial vaccine antigen, Ag85B-ESAT-6. The IC31 adjuvant, consisting of a vehicle based on the cationic peptide KLKL(5)KLK and the immunostimulatory oligodeoxynucleotide ODN1a signalling through the TLR9 receptor, was found to promote highly efficient Th1 responses. The combination of Ag85B-ESAT-6 and IC31 exhibited significant levels of protection in the mouse aerosol challenge model of tuberculosis and a detailed analysis of the immune response generated revealed the induction of CD4 T cells giving rise to high levels of IFN-gamma secretion. Furthermore, the combination of Ag85B-ESAT-6/IC31 was found to confer efficient protection in the guinea pig aerosol model of tuberculosis infection and is at present moving towards clinical testing. |
| Databáze: | OpenAIRE |
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