Mechanisms of Memory Impairment Induced by Orexin-A via Orexin 1 and Orexin 2 Receptors in Post-traumatic Stress Disorder Rats
| Autor: | Shilei Zheng, Fang Han, Lili Wen, Dan Han, Yuxiu Shi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Memory Dysfunction Lateral hypothalamus media_common.quotation_subject Morris water navigation task Hippocampus Stress Disorders Post-Traumatic 03 medical and health sciences Orexin-A 0302 clinical medicine Orexin Receptors Internal medicine mental disorders medicine Animals Humans Rats Wistar media_common Memory Disorders Orexins business.industry General Neuroscience digestive oral and skin physiology Appetite Rats Orexin 030104 developmental biology Endocrinology nervous system Hypothalamus business hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience. 432:126-136 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/j.neuroscience.2020.02.026 |
| Popis: | Post-traumatic stress disorder (PTSD) patients exhibit abnormal learning and memory. Axons from orexin neurons in the lateral hypothalamus innervate the hippocampus, modulating learning and memory via the orexin 1 and 2 receptors (OX1R and OX2R). However, the role of the orexin system in the learning and memory dysfunction observed in PTSD is unknown. This was investigated in the present study using PTSD animal model-single prolonged stress (SPS) rats. Spatial learning and memory in the rats were evaluated with the Morris water maze (MWM) test; changes in body weight and food intake were recorded to assess changes in appetite; and the expression of orexin-A and its receptors in the hypothalamus and hippocampus was examined and quantified by immunohistochemistry, western blotting and real-time PCR. The results showed that spatial memory was impaired and food intake was decreased in SPS rats; this was accompanied by downregulation of orexin-A in the hypothalamus and upregulation of OX1R and OX2R in the hippocampus and of OX1R in the hypothalamus. Intracerebroventricular administration of orexin-A improved spatial memory and enhanced appetite in SPS rats and partly reversed the increases in OX1R and OX2R levels in the hippocampus and hypothalamus. These results suggest that the orexin system plays a critical role in the memory and appetite dysfunction observed in PTSD. |
| Databáze: | OpenAIRE |
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