Oral Food Desensitization in Children With IgE-Mediated Cow's Milk Allergy: Immunological Changes Underlying Desensitization
| Autor: | Rosina López-Fandiño, Iván López-Expósito, Teresa Valbuena, Laura Perezábad, Elena Molina, Marta Reche |
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| Přispěvatelé: | European Commission, Ministerio de Educación, Cultura y Deporte (España) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine medicine.medical_treatment Immunology desensitization Cow's milk allergy 03 medical and health sciences 0302 clinical medicine Ige mediated children milk oral immunotherapy medicine Immunology and Allergy Cost action health care economics and organizations Desensitization (medicine) business.industry food and beverages humanities 030104 developmental biology Cow’s milk allergy 030228 respiratory system Original Article business |
| Zdroj: | Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid Consejería de Sanidad de la Comunidad de Madrid Allergy, Asthma & Immunology Research Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | [Purpose]: This study aimed to evaluate the safety and efficacy to induce clinical desensitization to cow's milk (CM) of an oral immunotherapy (OIT) protocol in a pediatric population with cow's milk allergy (CMA). In addition, the immune responses against β-casein, of peripheral blood mononuclear cells (PBMCs) from CMA patients, before and after the protocol were evaluated and compared to a nonallergic population. [Methods]: A group of 20 children with IgE-mediated CMA and 15 nonallergic children were recruited. Allergic subjects underwent an OIT protocol based on weekly doses of commercial semi-skimmed ultra-high temperature treated (UHT) CM, followed by a maintenance phase. Immune profiles and changes in all subjects were investigated by measuring Th1, Th2, and Treg cytokines, transcription factors, and specific IgE and IgG4 levels. [Results]: The CMOIT protocol enabled to desensitize 70% of the allergic patients. Successful OIT was accompanied by significant increases in casein-specific IgG4 levels, together with a reduction in the concentration of antigen-specific IgE and in IL-5, IL-13, and IL-10 production by β-casein-stimulated PBMCs. Baseline significant differences observed between allergic and nonallergic children in IL-13 and IL-5 levels were no longer found once the protocol had finished. [Conclusions]: The OIT protocol was safe and effective in inducing milk desensitization in 70% of the children with CMA, leading to alterations in their immune profiles toward a nonallergic phenotype. Ms. Perezábad, Dr. López-Fandiño and Dr. Molina are members of the COST action ImpARAS FA1402. Ms. Perezábad is in receipt of a Formación de Personal Universitario Ph.D. Grant from the Ministerio de Educación, Cultura y Deporte (Spain). |
| Databáze: | OpenAIRE |
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