Gua Lou Gui Zhi decoction attenuates post-stroke spasticity via the modulation of GABAB receptors
| Autor: | Zuanfang Li, Jingjie Mao, Lidian Chen, Xiaoqin Zhu, Ruhui Lin, Haixia Hu |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Spasm Cancer Research Ischemia Gene Expression Motor Activity Pharmacology GABAB receptor Biochemistry Neuroprotection Genetics medicine Animals Receptor Molecular Biology Stroke business.industry Cerebral infarction Cerebral Infarction medicine.disease Rats Disease Models Animal Neuroprotective Agents medicine.anatomical_structure Receptors GABA-B Oncology Cerebral cortex Apoptosis Molecular Medicine business Psychomotor Performance Drugs Chinese Herbal |
| Zdroj: | Molecular Medicine Reports. 12:5957-5962 |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2015.4207 |
| Popis: | The aim of the present study was to investigate the mechanisms underlying the neuroprotective and antispastic effects of Gua Lou Gui Zhi decoction (GLGZD) in a rat model of middle cerebral artery occlusion (MCAO). The MCAO rats were treated with GLGZD (14.3 g/kg body weight) once a day for a period of seven days. Neurological deficit scores and screen tests were analyzed every other day. Following treatment with GLGZD for 7 days, the ischemic infarct volume of the rat brains was measured using 2,3,5‑triphenyl tetrazolium chloride staining. Reverse transcription‑polymerase chain reaction was performed in order to determine the mRNA expression levels of γ‑amino butyric acid B (γ‑GABAB) receptor (R) in the cortical infarct region. Furthermore, the protein expression levels of GABAB R were detected in the cortical infarct region by western blot analysis. Following 7 days, treatment with GLGZD significantly ameliorated the neurological defects and cerebral infarction in the MCAO rats. In addition, treatment with GLGZD ameliorated motor performance in the MCAO rats, as determined by screen tests. Furthermore, GLGZD was able to upregulate the mRNA and protein expression levels of GABAB1 R and GABAB2 R in the ischemic cerebral cortex. The results of the present study suggested that GLGZD may exert neuroprotective and antispastic effects in a cerebral ischemia model, through upregulating the expression of GABAB R. |
| Databáze: | OpenAIRE |
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