Circulating fetuin-A levels are not affected by short and long-term energy deprivation and/or by leptin administration
| Autor: | Bindiya Thakkar, Janice J. Hwang, John P. Chamberland, Christos S. Mantzoros |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
Leptin Male medicine.medical_specialty Time Factors alpha-2-HS-Glycoprotein Endocrinology Diabetes and Metabolism Adipose tissue Placebo Article Metreleptin chemistry.chemical_compound Endocrinology Thinness In vivo Internal medicine medicine Humans RNA Messenger Aged Caloric Restriction Leptin Deficiency business.industry digestive oral and skin physiology Middle Aged Fetuin chemistry Adipose Tissue Liver Female business Fetal bovine serum |
| Zdroj: | Metabolism: clinical and experimental. 63(6) |
| ISSN: | 1532-8600 |
| Popis: | Objective Fetuin-A may mediate cross-talk between the liver and adipose tissue. We studied the physiologic regulation of fetuin-A and explored its potential regulation by leptin. Design and Methods Fetuin-A levels were measured in three interventional studies as well as in in vitro experiments. Study 1: 15 lean subjects received placebo or physiologic replacement-dose recombinant human leptin (metreleptin) following short term complete caloric deprivation to induce severe hypoleptinemia; Study 2: 7 women with relative leptin deficiency due to strenuous exercise or low weight received 3 months of metreleptin; Study 3: 17 women with relative leptin deficiency were randomized to receive metreleptin or placebo over 9 months. In study 4 human hepatoma Hep G2 cells were treated with leptin. Fetuin-A mRNA expression and secretion were measured. Results Complete caloric deprivation significantly decreased leptin but had no effect on fetuin-A levels. Normalizing leptin levels with metreleptin in hypoleptinemic subjects had no effect on circulating fetuin-A levels. Leptin treatment had no effect on fetuin-A mRNA expression and secretion in vitro. Conclusions Circulating fetuin-A levels are not affected by short and long-term energy deprivation. Furthermore, both in vivo and in vitro experiments confirm that fetuin-A is not regulated by leptin. |
| Databáze: | OpenAIRE |
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