Selected reaction monitoring for colorectal cancer diagnosis using a set of five serum peptides identified by BLOTCHIP
| Autor: | Yuji Naito, Osamu Dohi, Eigo Otsuji, Yasuko Hirai, Yasuki Higashimura, Lyang-Ja Lee, Takeshi Ishikawa, Hideyuki Konishi, Katsura Mizushima, Kazuhiko Uchiyama, Yoshito Itoh, Tomohisa Takagi, Kazuhiro Katada, Asada Kyoichi, Tetsuya Okayama, Osamu Handa, Nobuaki Yagi, Naohisa Yoshida, Kenji Tanaka, Yoshiaki Kuriu, Daisuke Nonaka, Masayoshi Nakanishi, Kazuhiro Kamada |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Oncology Male Proteomics medicine.medical_specialty Colorectal cancer Colonic Polyps Diagnosis Differential 03 medical and health sciences 0302 clinical medicine Surgical oncology Internal medicine Biomarkers Tumor Medicine Humans Stage (cooking) neoplasms Early Detection of Cancer Aged Neoplasm Staging Aged 80 and over Blood Specimen Collection business.industry Gastroenterology Cancer Hepatology Middle Aged medicine.disease digestive system diseases Colorectal surgery 030104 developmental biology ROC Curve 030220 oncology & carcinogenesis Case-Control Studies Cohort Biomarker (medicine) Female Reagent Kits Diagnostic business Colorectal Neoplasms Peptides |
| Zdroj: | Journal of gastroenterology. 53(11) |
| ISSN: | 1435-5922 |
| Popis: | Colorectal cancer (CRC) is one of the most predominant types of cancer, and it is the fourth most common cause of cancer-related death and it is important to diagnose CRC in early stage to decrease the mortality by CRC. In our previous study, we identified a combination of five peptides as a biomarker candidate to diagnose CRC by BLOTCHIP®-MS analysis using a set of healthy control subjects and CRC patients (stage II–IV). The aim of the present study was to validate the serum biomarker peptides reported in our previous study using a second cohort and to establish their potential usefulness in CRC diagnosis. A total of 56 patients with CRC (n = 14 each of stages I–IV), 60 healthy controls, and 60 patients with colonic adenoma were included in this study. The five peptides were extracted and analyzed by selected reaction monitoring using ProtoKey® Colorectal Cancer Risk Test Kit (Protosera, Inc., Amagasaki, Japan). The results clearly showed that the four CRC groups, stages I–IV, could be sufficiently discriminated from the control group and colonic polyp group. This five-peptide set could identify CRC at each stage compared to the control population in this validation cohort, including those with early-stage disease. The AUC values for each stage of CRC compared to the control population were 0.779, 0.946, 0.852, and 0.973 for stages I, II, III, and IV, respectively. In this case–control validation study, we confirmed high diagnostic performance for CRC using five peptides that were identified in our previous study as serum biomarker candidates for the detection of CRC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink