PROTEIN SYNTHESIS AND GENE EXPRESSION IN TRANSPLANTED AND POSTISCHEMIC LIVERS
| Autor: | Gaetano Cairo, Antonio Piazzini-Albani, Carmela Pappalardo, Lorenza Tacchini, Stefano Gatti, Aldo Bernelli-Zazzera, Luisa Schiaffonati |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Pathology medicine.medical_specialty Transcription Genetic medicine.medical_treatment Gene Expression Biology Liver transplantation Andrology In vivo Gene expression Protein biosynthesis medicine Animals Electrophoresis Gel Two-Dimensional Viaspan RNA Messenger Rats Wistar Cryopreservation chemistry.chemical_classification Transplantation Albumin Proteins Blotting Northern Liver Transplantation Rats Liver chemistry Transferrin Protein Biosynthesis Reperfusion Injury |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/00007890-199305000-00004 |
| Popis: | The expression of some genes has been comparatively studied in transplanted rat liver and in liver reperfused after ischemia in situ. Experiments on protein synthesis by tissue slices from cold-stored or transplanted livers show that rat livers that retain a good capacity for protein synthesis during storage undergo a profound impairment in the capacity for protein synthesis during the first hours after transplantation. This recovers in the following hours. There is never any indication of synthesis of stress proteins, and of hsp 70 in particular. The steady-state level of mRNAs for albumin, transferrin, and beta-actin, which are well expressed in reperfused postischemic livers in vivo, are reduced early after transplantation and recover only many hours later. Run-on analysis shows that an early defect in transcription and a partial recovery of this process later on are responsible for these changes. The steady-state levels of the same mRNAs are well maintained in donor livers preserved in University of Wisconsin solution for at least 12 hr, and less satisfactorily in Euro-Collins solution. Results of run-on analysis parallel the data on mRNA levels. The behavior of these mRNAs is, therefore, clearly different in reperfused and transplanted liver. The early stages of liver transplantation seem to be characterized by a depressed capacity of gene expression, without the reactive phenomenon of activation of stress protein genes that occurs in reperfused postischemic livers. |
| Databáze: | OpenAIRE |
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