Molecular cloning and functional analysis of cDNA encoding a rat leukemia inhibitory factor: towards generation of pluripotent rat embryonic stem cells
Autor: | Hiroshige Nakano, Hiroyasu Baba-Toriyama, Masaaki Terada, Yasushi Takahama, Hiroki Sasaki, Hiroyasu Konishi, Takahiro Ochiya |
---|---|
Rok vydání: | 1998 |
Předmět: |
endocrine system
Cancer Research DNA Complementary Cellular differentiation Molecular Sequence Data Biology Leukemia Inhibitory Factor Mice Pregnancy Complementary DNA Tumor Cells Cultured Genetics Animals Amino Acid Sequence RNA Messenger Cloning Molecular Induced pluripotent stem cell Molecular Biology reproductive and urinary physiology Lymphokines Base Sequence Interleukin-6 urogenital system Stem Cells Embryo Mammalian Embryonic stem cell Molecular biology Growth Inhibitors Rats Inbred F344 In vitro Rats Cell culture embryonic structures Female Stem cell Leukemia inhibitory factor hormones hormone substitutes and hormone antagonists |
Zdroj: | Oncogene. 16:3189-3196 |
ISSN: | 1476-5594 0950-9232 |
DOI: | 10.1038/sj.onc.1201826 |
Popis: | Embryonic stem (ES) cells are pluripotent cell lines established directly from the early embryo. Maintenance of the stem-cell phenotype of ES cells in vitro requires the presence of a feeder layer of fibroblasts or of a soluble factor, differentiation inhibitory activity (DIA) such as leukemia inhibitory factor (LIF). Here we report the cloning of complete rat LIF cDNA and its nucleotide sequence so as to facilitate studies of rat ES cell technologies on tumor biology. The nucleotide sequence of the rat LIF cDNA indicated that the rat LIF has 91% amino acid sequence identity with murine LIF. The cloned rat LIF cDNA has a putative biological activity as a differentiation-inducing factor on the murine myeloid leukemia cell line M1 cells. Culture supernatant of the rat LIF cDNA-transduced rat fibroblast cell line could maintain the stem-cell phenotype of rat ES cells which showed alkaline phosphatase activity, and this effect was much stronger than that by murine LIF. The availability of rat LIF which shows DIA will assist the in vitro analysis of rat ES cells, and culture of these cells is a route for the generation of gene targeting in rat. |
Databáze: | OpenAIRE |
Externí odkaz: |