Effects on Metabolism in Astrocytes Caused by cGAMP, Which Imitates the Initial Stage of Brain Metastasis
| Autor: | Hiroki Tanaka, Hiromi Sato, Masataka Kogane, Hiroto Hatakeyama, Hidetaka Akita, Akihiro Hisaka, Toya Okawa, Momoko Goto, Kurumi Hara, Moe Kikuchi, Daiki Shirane |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
Central nervous system Primary Cell Culture metabolome shift glutamate Biology Catalysis Article Malignant transformation Inorganic Chemistry astrocyte medicine Animals metastatic brain tumor Secretion Physical and Theoretical Chemistry Neoplasm Metastasis Rats Wistar Biology (General) Molecular Biology QD1-999 Spectroscopy gamma-Aminobutyric Acid Brain Neoplasms Organic Chemistry Glutamate receptor Gap junction General Medicine Computer Science Applications Cell biology Glutamine Chemistry medicine.anatomical_structure Glucose Astrocytes cGAMP Second messenger system Nucleotides Cyclic Astrocyte |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 16 International Journal of Molecular Sciences, Vol 22, Iss 9028, p 9028 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22169028 |
| Popis: | The second messenger 2′3′-cyclic-GMP-AMP (cGAMP) is thought to be transmitted from brain carcinomas to astrocytes via gap junctions, which functions to promote metastasis in the brain parenchyma. In the current study, we established a method to introduce cGAMP into astrocytes, which simulates the state of astrocytes that have been invaded by cGAMP around tumors. Astrocytes incorporating cGAMP were analyzed by metabolomics, which demonstrated that cGAMP increased glutamate production and astrocyte secretion. The same trend was observed for γ-aminobutyric acid (GABA). Conversely, glutamine production and secretion were decreased by cGAMP treatment. Due to the fundamental role of astrocytes in regulation of the glutamine–glutamate cycle, such metabolic changes may represent a potential mechanism and therapeutic target for alteration of the central nervous system (CNS) environment and the malignant transformation of brain carcinomas. |
| Databáze: | OpenAIRE |
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