Oleic acid induces migration through a FFAR1/4, EGFR and AKT-dependent pathway in breast cancer cells
| Autor: | Eduardo Perez Salazar, Cleofas Marcial-Medina, Christian Gonzalez-Reyes, Pedro Cortes-Reynosa, Alejandra Ordoñez-Moreno |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Endocrinology Diabetes and Metabolism AKT1 AKT2 migration lcsh:Diseases of the endocrine glands. Clinical endocrinology 03 medical and health sciences breast cancer 0302 clinical medicine Endocrinology Free fatty acid receptor 1 oleic acid (OA) Internal Medicine 12-LOX Medicine Protein kinase B PI3K/AKT/mTOR pathway lcsh:RC648-665 business.industry Research Cell migration invasion 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Signal transduction business Energy source |
| Zdroj: | Endocrine Connections, Vol 8, Iss 3, Pp 252-265 (2019) Endocrine Connections |
| ISSN: | 2049-3614 |
| DOI: | 10.1530/ec-18-0543 |
| Popis: | Free fatty acids (FFAs) are an energy source, and induce activation of signal transduction pathways that mediate several biological processes. In breast cancer cells, oleic acid (OA) induces proliferation, matrix metalloproteinase-9 (MMP-9) secretion, migration and invasion. However, the signal transduction pathways that mediate migration and invasion induced by OA in breast cancer cells have not been studied in detail. We demonstrate here that FFAR1 and FFAR4 mediate migration induced by OA in MDA-MB-231 and MCF-7 breast cancer cells. Moreover, OA induces migration, invasion, AKT1 and AKT2 activation, 12-LOX secretion and an increase of NFκB-DNA binding activity in breast cancer cells. Cell migration requires FFAR1, FFAR4, EGFR, AKT and PI3K activity, whereas invasion is mediated though a PI3K/Akt-dependent pathway. Furthermore, OA promotes relocalization of paxillin to focal contacts and it requires PI3K and EGFR activity, whereas NFκB-DNA binding activity requires PI3K and AKT activity. |
| Databáze: | OpenAIRE |
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