Decreased microbial co-occurrence network stability and SCFA receptor level correlates with obesity in African-origin women
| Autor: | Xiang Gao, David E. Nelson, Brian T. Layden, Lara R. Dugas, Neil Gottel, Jacob Plange-Rhule, Laquita Brown, Stephanie Kliethermes, Evelyn Toh, Jack A. Gilbert, Medha Priyadarshini, Amy Luke, Beatriz Penalver Bernabe, Jun Sun, Seo J. Park, Na Fei, Qunfeng Dong |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Volatile lcsh:Medicine Gut flora Ghana Oral and gastrointestinal Body Mass Index Receptors G-Protein-Coupled Butyric acid South Africa chemistry.chemical_compound Receptors Food science lcsh:Science Adiposity African Americans 2. Zero hunger chemistry.chemical_classification Multidisciplinary biology Fatty Acids Fecal Microbiota Transplantation Parabacteroides Cell Surface Female Adult 030106 microbiology Receptors Cell Surface Diet High-Fat Article G-Protein-Coupled 03 medical and health sciences Clinical Research parasitic diseases medicine Humans Obesity Metabolic and endocrine Feces Nutrition lcsh:R Lachnospiraceae Fatty acid Feeding Behavior Fatty Acids Volatile biology.organism_classification medicine.disease Diet Gastrointestinal Microbiome Black or African American High-Fat 030104 developmental biology chemistry lcsh:Q Bacteroides Digestive Diseases |
| Zdroj: | Scientific Reports Scientific reports, vol 8, iss 1 Scientific Reports, Vol 8, Iss 1, Pp 1-17 (2018) |
| ISSN: | 2045-2322 |
| Popis: | We compared the gut microbial populations in 100 women, from rural Ghana and urban US [50% lean (BMI 2) and 50% obese (BMI ≥ 30 kg/m2)] to examine the ecological co-occurrence network topology of the gut microbiota as well as the relationship of short chain fatty acids (SCFAs) with obesity. Ghanaians consumed significantly more dietary fiber, had greater microbial alpha-diversity, different beta-diversity, and had a greater concentration of total fecal SCFAs (p-value Bacteroides uniformis was significantly more abundant in lean women, irrespective of country (FDR corrected p Ruminococcus callidus, Prevotella copri, and Escherichia coli, and smaller proportions of Lachnospiraceae, Bacteroides and Parabacteroides. Lean Ghanaians had a significantly greater abundance of predicted microbial genes that catalyzed the production of butyric acid via the fermentation of pyruvate or branched amino-acids, while obese Ghanaians and US women (irrespective of BMI) had a significantly greater abundance of predicted microbial genes that encoded for enzymes associated with the fermentation of amino-acids such as alanine, aspartate, lysine and glutamate. Similar to lean Ghanaian women, mice humanized with stool from the lean Ghanaian participant had a significantly lower abundance of family Lachnospiraceae and genus Bacteroides and Parabacteroides, and were resistant to obesity following 6-weeks of high fat feeding (p-value Ffa) receptor Ffa2, in spite of similar fecal SCFAs concentrations. We demonstrate that the association between obesity resistance and increased predicted ecological connectivity and stability of the lean Ghanaian microbiota, as well as increased local SCFA receptor level, provides evidence of the importance of robust gut ecologic network in obesity. |
| Databáze: | OpenAIRE |
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