Dynamics of heart differentiation, visualized utilizing heart enhancer elements of the Drosophila melanogaster bHLH transcription factor Hand
| Autor: | Stefanie Albrecht, Julia Sellin, Verena Kölsch, Achim Paululat |
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| Rok vydání: | 2006 |
| Předmět: |
Cell type
Embryo Nonmammalian media_common.quotation_subject Green Fluorescent Proteins Biology Genes Reporter Basic Helix-Loop-Helix Transcription Factors Genetics Animals Drosophila Proteins Metamorphosis Heart formation Molecular Biology Transcription factor media_common Microscopy Video Heart development Embryogenesis Metamorphosis Biological Pupa Gene Expression Regulation Developmental Cell Differentiation Heart Embryo biology.organism_classification Immunohistochemistry Cell biology DNA-Binding Proteins Drosophila melanogaster Enhancer Elements Genetic Lac Operon Larva Insect Proteins Transcription Factors Developmental Biology |
| Zdroj: | Gene Expression Patterns. 6:360-375 |
| ISSN: | 1567-133X |
| DOI: | 10.1016/j.modgep.2005.09.012 |
| Popis: | Drosophila melanogaster has become one of the important model systems to investigate the development and differentiation of the heart. After 24 h after egg deposition (h AED), a simple tube-like organ is formed, consisting of essentially only two cell types, the contractile cardioblasts and non-myogenic pericardial cells. In contrast to the detailed knowledge of heart formation during embryogenesis, only a few studies deal with later changes in heart morphology and/or function. This is mainly due to the difficulties to carry out whole mount stainings in later stages without complicated dissections or treatments of the cuticle and puparium. In this paper we describe the identification of a hand genomic region, which is fully sufficient to drive GFP expression in heart cells of embryos, larvae, and adults. This serves as an initial step to understand the position of hand in the early regulatory network in heart development. Furthermore, we demonstrate that our newly created GFP reporter line is extremely useful to study postembryonic heart differentiation. For the first time we document heart differentiation in living animals throughout all developmental stages of Drosophila melanogaster, including embryogenesis, all three larval stages, metamorphosis, and the adult life with respect to pericardial cells and cardiomyocytes. |
| Databáze: | OpenAIRE |
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