Dietary Ingestion of Calories and Micronutrients Modulates the DNA Methylation Profile of Leukocytes from Older Individuals
Autor: | Marcus Vinicius de Matos Gomes, Gislaine Garcia Pelosi, Karen Barros Parron Fernandes, J. Passador, Rejane D. Neves-Souza, L.V. Toffoli |
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Rok vydání: | 2018 |
Předmět: |
Male
0301 basic medicine Vitamin medicine.medical_specialty Calorie medicine.medical_treatment Medicine (miscellaneous) 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Leukocytes medicine Vitamin D and neurology Humans Micronutrients Epigenetics Aged Aged 80 and over Nutrition and Dietetics business.industry Vitamin E Global DNA Methylation Profile DNA Methylation Middle Aged Micronutrient Diet 030104 developmental biology Endocrinology chemistry 030220 oncology & carcinogenesis DNA methylation Female Geriatrics and Gerontology Energy Intake business |
Zdroj: | The journal of nutrition, health & aging. 22:1281-1285 |
ISSN: | 1760-4788 1279-7707 |
Popis: | Several lines of evidence from the last decade support the connection between nutrition and epigenetic mechanisms. In the present study we evaluated the impact of the daily dietary intake of calories and the micronutrients vitamin A, D, B1, B2, B5, C, E, copper, calcium, phosphorus, iron, iodine, selenium, manganese, potassium and sodium on the global DNA methylation profile of blood cells from older individuals. The study enrolled 126 physically independent elderly of both sexes (60 men and 66 women). For the molecular analysis, DNA samples were extracted from leukocytes and global DNA methylation was evaluated using a high throughput Elisa-based method. Correlations between global DNA methylation and the daily intake of calorie or micronutrients were evaluated using Prism5 GraphPad Software. A statistically significant correlation was observed between global DNA methylation and the daily caloric value (p=0.019, r=-0.21), and the intake of vitamin A (p=0.03, r=-0.18), Vitamin E (p=0.027, r=-0.20) and copper (p=0.04, r=-0.18). No correlation was observed between global DNA methylation and the daily intake of vitamin D, B1, B2, B5, C, calcium, phosphorus, iron, iodine, selenium, manganese and potassium (p>0.05). Our data demonstrate that the daily intake of calories or the micronutrients vitamin A, vitamin E and copper can potentially modulate the global DNA methylation profile of leukocytes in older adults and corroborate the notion of nutritional influences on epigenetic mechanisms. |
Databáze: | OpenAIRE |
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