Characterization of NR13-related human cell death regulator, Boo/Diva, in normal and cancer tissues
| Autor: | Paul E. Neiman, Connie P. Matthews, Ruey-min Lee, James K. McDougall, Jun Chen |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Programmed cell death
Green Fluorescent Proteins Molecular Sequence Data Biophysics Mitochondrion Kidney Transfection Biochemistry Cell Line Green fluorescent protein Avian Proteins HeLa Structural Biology Neoplasms Genetics Humans Amino Acid Sequence Gene Cell Death biology Ovary HEK 293 cells Chromosome Mapping biology.organism_classification Immunohistochemistry Molecular biology Diva Luminescent Proteins Liver Proto-Oncogene Proteins c-bcl-2 Apoptosis Female Sequence Alignment |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1520:187-194 |
| ISSN: | 0167-4781 |
| DOI: | 10.1016/s0167-4781(01)00268-8 |
| Popis: | Mouse Boo/Diva is an ovary-specific member of the Bcl-2 family identified through homology with the avian cell death antagonist NR13. We identified a human orthologue of Boo/Diva, which is highly conserved between mouse and human and related to avian NR13. Human Boo/Diva is also expressed in human liver and kidney in addition to the ovary. We found that green fluorescence protein (EGFP)-tagged Boo/Diva was not exclusively localized to mitochondria before the induction of apoptosis. However, EGFP-Boo/Diva translocated to mitochondria in the process of apoptosis induced by vincristine, a microtubule-interfering agent. Overexpression of human Boo/Diva promoted cell death in HeLa and 293 cells. The cell death antagonist Bcl-XL interacts with Boo, but is unable to protect 293 cells from Boo/Diva-induced cell death. Finally, we mapped human Boo/Diva to chromosome 15q21, a locus known to be related to human cervical cancer. Moreover, we found that genomic DNAs of three of 24 human cervical cancer samples display deletions within their Boo/Diva genes. This result suggests a role for human Boo/Diva in the pathogenesis of cervical cancer. |
| Databáze: | OpenAIRE |
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