An investigation on the role of nitric oxide in the modulation of the binding characteristics of various radioligands of GABAA receptors by 5α-pregnan-3α-ol-20-one in the rat brain regions
| Autor: | Maharaj K. Ticku, Ashok K. Mehta |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
medicine.medical_specialty Neuroactive steroid Hippocampus Pregnanolone Arginine Nitric Oxide Nitric oxide Rats Sprague-Dawley Radioligand Assay chemistry.chemical_compound Cerebellum Internal medicine medicine Animals Enzyme Inhibitors GABA Modulators Molecular Biology Cerebral Cortex biology GABAA receptor General Neuroscience Brain Receptors GABA-A Rats Nitric oxide synthase NG-Nitroarginine Methyl Ester medicine.anatomical_structure Endocrinology nervous system chemistry Muscimol Cerebral cortex biology.protein Neurology (clinical) Flunitrazepam Developmental Biology medicine.drug |
| Zdroj: | Brain Research. 832:164-167 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/s0006-8993(99)01410-9 |
| Popis: | Chronic administration of Nω-nitro- l -arginine methyl ester ( l -NAME), an inhibitor of nitric oxide synthase, diminished the ability of 5α-pregnan-3α-ol-20-one, a neurosteroid, to potentiate the [ 3 H ]muscimol (5 nM) binding in the rat hippocampus but not in the cerebellum or cerebral cortex. This effect of NAME was stereospecific and susceptible to reversal by the pre-treatment of rats with l -arginine. However, chronic administration of l -NAME did not affect the modulation of the [ 3 H ]flunitrazepam (2 nM) or [ 35 S ]TBPS (4 nM) binding by the neurosteroid in any of the brain regions investigated in this study. These results suggest that nitric oxide may be involved in some of the effects of neurosteroids in hippocampus. |
| Databáze: | OpenAIRE |
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