Vegetative compatibility and heterokaryon formation between different isolates of Colletotrichum lindemuthianum by using the nit mutant system
| Autor: | Maria Cristina Mendes-Costa, Camila Rodrigues de Carvalho |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Genetics
Heterokaryon anthracnose education.field_of_study biology Colletotrichum lindemuthianum fungi Mutant Population lcsh:QR1-502 heterokaryosis nit mutants Genetics and Molecular Microbiology biology.organism_classification Microbiology lcsh:Microbiology Parasexual cycle Spore Complementation VCG Botany Genetic variability education Research Paper |
| Zdroj: | Brazilian Journal of Microbiology Brazilian Journal of Microbiology, Volume: 42, Issue: 1, Pages: 346-353, Published: MAR 2011 Brazilian Journal of Microbiology, Vol 42, Iss 1, Pp 346-353 (2011) Brazilian Journal of Microbiology v.42 n.1 2011 Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
| ISSN: | 1517-8382 |
| DOI: | 10.1590/s1517-83822011000100044 |
| Popis: | Colletotrichum lindemuthianum, the causative agent of bean anthracnose, is one of the most common pathogens leading to expressive damage to plants beyond presenting noticeable variability. The knowledge on vegetative compatibility groups (VCGs) is of particular interest in asexual fungi as they subdivide the population in groups that can exchange genetic information via heterokaryosis and the parasexual cycle. Among the techniques used in studies about vegetative compatibility groups, the obtainment of nit mutants is apparent. This paper is aimed at obtaining heterokaryons between different isolates of C. lindemuthianum, grouping them in VCGs and evaluating their genetic variability by using the nit mutants system. Nit mutants were obtained from 20 single spore isolates. The mutants were phenotypically classified and paired for complementation and formation of heterokaryons so as to group them in VCGs. Seventeen mutants from the different phenotypic-rates were recovered: nit1, nit2, nit3 and nitM. At the same time, 10 mutants were selected for pairing and division of the anastomosis groups. Nine heterokaryons were obtained and the isolates were divided into 9 vegetative compatibility groups. In the combinations for the formation of anastomosis, 31 compatible combinations and 24 incompatible combinations were observed. It was concluded that the methodology used to select nit mutants in C. lindemuthianum made it possible to determine the vegetative compatibility groups and that such a technique was adequate to prove genetic variability. |
| Databáze: | OpenAIRE |
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