Aliicoccus persicus gen. nov., sp. nov., a halophilic member of the Firmicutes isolated from a hypersaline lake
| Autor: | Mohammad Ali Amoozegar, Maryam Didari, Mahdi Moshtaghi Nikou, Antonio Ventosa, Maryam Bagheri, Peter Schumann, Cristina Sánchez-Porro, Ali Makhdoumi-Kakhki |
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| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Salinity Firmicutes Molecular Sequence Data Peptidoglycan Iran Staphylococcaceae Microbiology Cell Wall RNA Ribosomal 16S Nosocomiicoccus Phospholipids Phylogeny Ecology Evolution Behavior and Systematics Salinicoccus Base Composition biology Fatty Acids Jeotgalicoccus Vitamin K 2 Sequence Analysis DNA General Medicine Hypersaline lake Ribosomal RNA biology.organism_classification 16S ribosomal RNA Halophile Bacterial Typing Techniques Lakes Water Microbiology |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 64:1964-1969 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.058545-0 |
| Popis: | A novel Gram-staining-positive, moderately halophilic bacterium, designated strain A76T, was isolated from a brine sample of the hypersaline lake Aran-Bidgol in Iran. Cells were strictly aerobic, coccus-shaped, non-motile, non-sporulating, and catalase- and oxidase-positive. Strain A76T grew between pH 7.0 and 10.0 (optimal growth at pH 8.0), between 20 and 45 °C (optimal growth at 35 °C) and at salinities of 0.5 to 12.5 % (w/v) NaCl (optimal growth at 7.5 %, w/v, NaCl). On the basis of 16S rRNA gene sequence analysis, strain A76T was shown to belong to the phylum Firmicutes with sequence similarities of 94.1, 93.1 and 91.1 %, to the type species of the genera Jeotgalicoccus , Salinicoccus and Nosocomiicoccus , respectively. The DNA G+C content of this new isolate was 38.8 mol%. The major cellular fatty acids of strain A76T were anteiso-C15 : 0 and iso-C15 : 0, and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, a glycolipid, an unknown lipid and two unknown phospholipids. The isoprenoid quinones were MK-6 (94 %), MK-5 (3 %) and MK-7 (3 %). The amino acid constituents of the cell wall were Lys, Asp, Gly, Glu and Ala. The physiological, biochemical and phylogenetic differences between strain A76T and type strains of taxa with validly published names suggest that this strain represents a novel species in a novel genus within the family Staphylococcaceae , for which the name Aliicoccus persicus gen. nov., sp. nov. is proposed. The type strain of Aliicoccus persicus is strain A76T ( = CECT 8508T = DSM 28306T = IBRC-M 10081T). |
| Databáze: | OpenAIRE |
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