Loss of a highly conserved sterile alpha motif domain gene (WEEP) results in pendulous branch growth in peach trees
| Autor: | Amy Tabb, Chinnathambi Srinivasan, Ralph Scorza, Zhongchi Liu, Thierry Pascal, Toto Hadiarto, Wanpeng Wang, Courtney A. Hollender, Chris Dardick |
|---|---|
| Přispěvatelé: | Michigan State University [East Lansing], Michigan State University System, Department of Horticulture, University of Wisconsin-Madison, Génétique et Amélioration des Fruits et Légumes (GAFL), Institut National de la Recherche Agronomique (INRA), Indonesian Center for Agricultural Land Resource Reseach and Development, University of Maryland [College Park], University of Maryland System, US Department of Agriculture National Institute of Food and Agriculture Agriculture and Food Research Initiative 10891264, National Science Foundation 1339211SBI-0521250, European Project: 265582,EC:FP7:KBBE,FP7-KBBE-2010-4,FRUIT BREEDOMICS(2011) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine [SDV.SA]Life Sciences [q-bio]/Agricultural sciences Multidisciplinary fungi Gravitropism 15. Life on land Biology 01 natural sciences gravitropism SAM domain peach Weep 03 medical and health sciences Prunus 030104 developmental biology weeping Lateral shoot Shoot Botany tree architecture Habit (biology) Phloem Sterile alpha motif 010606 plant biology & botany |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2018, 115 (20), pp.E4690-E4699. ⟨10.1073/pnas.1704515115⟩ Proceedings of the National Academy of Sciences of the United States of America, 2018, 115 (20), pp.E4690-E4699. ⟨10.1073/pnas.1704515115⟩ |
| ISSN: | 0027-8424 1091-6490 |
| DOI: | 10.1073/pnas.1704515115⟩ |
| Popis: | International audience; Plant shoots typically grow upward in opposition to the pull of gravity. However, exceptions exist throughout the plant kingdom. Most conspicuous are trees with weeping or pendulous branches. While such trees have long been cultivated and appreciated for their ornamental value, the molecular basis behind the weeping habit is not known. Here, we characterized a weeping tree phenotype in Prunus persica (peach) and identified the underlying genetic mutation using a genomic sequencing approach. Weeping peach tree shoots exhibited a downward elliptical growth pattern and did not exhibit an upward bending in response to 90 degrees reorientation. The causative allele was found to be an uncharacterized gene, Ppa013325, having a 1.8-Kb deletion spanning the 5' end. This gene, dubbed WEEP, was predominantly expressed in phloem tissues and encodes a highly conserved 129-amino acid protein containing a sterile alpha motif (SAM) domain. Silencing WEEP in the related tree species Prunus domestica (plum) resulted in more outward, downward, and wandering shoot orientations compared to standard trees, supporting a role for WEEP in directing lateral shoot growth in trees. This previously unknown regulator of branch orientation, which may also be a regulator of gravity perception or response, provides insights into our understanding of how tree branches grow in opposition to gravity and could serve as a critical target for manipulating tree architecture for improved tree shape in agricultural and horticulture applications. |
| Databáze: | OpenAIRE |
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