Basal omega-3 fatty acid status affects fatty acid and oxylipin responses to high-dose n3-HUFA in healthy volunteers
| Autor: | Mark K. Larson, Gregory C. Shearer, Theresa L. Pedersen, John W. Newman, Alison H. Keenan, Kristi Fillaus |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Blood Platelets Male medicine.medical_specialty Erythrocytes QD415-436 Biology Drug Prescriptions Biochemistry Young Adult Basal (phylogenetics) Endocrinology Internal medicine Fatty Acids Omega-3 targeted metabolomics medicine Humans Oxylipins baseline status Omega 3 fatty acid chemistry.chemical_classification omega-3 fatty acids Dose-Response Relationship Drug Esterification Maintenance dose Fatty acid Cell Biology Middle Aged Oxylipin Red blood cell Dose–response relationship medicine.anatomical_structure chemistry Health highly unsaturated fatty acid Basal metabolic rate Female Basal Metabolism Patient-Oriented and Epidemiological Research |
| Zdroj: | Journal of Lipid Research, Vol 53, Iss 8, Pp 1662-1669 (2012) |
| ISSN: | 0022-2275 |
| DOI: | 10.1194/jlr.p025577 |
| Popis: | A subject's baseline FA composition may influence the ability of dietary highly unsaturated omega-3 FAs (n3-HUFA) to change circulating profiles of esterified FAs and their oxygenated metabolites. This study evaluates the influence of basal n3-HUFA and n3-oxylipin status on the magnitude of response to n3-HUFA consumption. Blood was collected from fasting subjects (n = 30) before and after treatment (4 weeks; 11 ± 2 mg/kg/day n3-HUFA ethyl esters). Esterified FAs were quantified in erythrocytes, platelets, and plasma by GC-MS. Esterified oxylipins were quantified in plasma by LC-MS/MS. Treatment with n3-HUFAs increased n3-HUFAs and decreased n6-HUFAs in all reservoirs and increased plasma n3-oxylipins without significantly changing n6-oxylipin concentrations. As subject basal n3-HUFAs increased, treatment-associated changes decreased, and this behavior was reflected in the percentage of 20:5n3 + 22:6n3 in red blood cell membrane FAs (i.e., the omega-3 index). To maintain an omega-3 index of 8% and thus reduce cardiovascular disease risk, our analyses suggest a maintenance dose of 7 mg/kg/day n3-HUFA ethyl esters for a 70-kg individual. These results suggest that the basal n3 index may have clinical utility to establish efficacious therapeutic experimental feeding regimens and to evaluate the USDA Dietary Guidelines recommendations for n3-HUFA consumption. |
| Databáze: | OpenAIRE |
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