HLA typing of Epstein-Barr virus transformed lymphoblastoid cell lines (LCL)
| Autor: | V. Stinchcombe, T. J. Jones, B A Bradley |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Herpesvirus 4
Human Time Factors Cell Survival Immunology Spleen Human leukocyte antigen medicine.disease_cause Lymphocyte Activation Biochemistry Virus Cell Line HLA Antigens Genetics medicine Immunology and Allergy Humans Typing medicine.diagnostic_test biology Histocompatibility Testing General Medicine Cell Transformation Viral Cytotoxicity Tests Immunologic Epstein–Barr virus Virology medicine.anatomical_structure Phenotype Cell culture biology.protein Antibody Tissue typing |
| Zdroj: | Tissue antigens. 26(3) |
| ISSN: | 0001-2815 |
| Popis: | The application of standard tissue typing techniques to cells other than peripheral blood lymphocytes has been accompanied by the problem of extra reactions. This applies as well to Epstein-Barr virus transformed lymphoblastoid cell lines (LCL) as to leukemic cells and human spleen cells. These extra reactions are attributable to additional antibodies in the typing sera which are not apparent under standard conditions with PBLs. Two types are described: Type 1 extras, which becomes apparent after longer incubation times and are attributed to weak antibodies and type 2 extras which are apparent after shorter incubation times and are attributed to subpopulation specific or differentiation antigens. Technical modifications are proposed by which these extras can be circumvented. They include: Only start typing when cells have been cultured for 2 to 3 days. Remove dead cells by spinning over standard ficoll-hypaque or 11% triosil. Use shorter incubation times. Avoid using sera that give too many type 2 extras. In this way phenotypes can be accurately identified on LCL's obtained from kidney transplant donors and recipients. When LCL's were compared with their matching PBL, HLA phenotypes were concordant in 87% of cases for HLA-A, 90% for HLA-B, 81% for HLA-C and 70% for HLA-DR. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text