Trojan horse monocyte-mediated delivery of conjugated polymer nanoparticles for improved photodynamic therapy of glioblastoma
| Autor: | Pilar López-Larrubia, Lucía Beaugé, Rodrigo E. Palacios, Viviana Rivarola, Luis Exequiel Ibarra, Carlos A. Chesta, Nuria Arias-Ramos |
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| Rok vydání: | 2020 |
| Předmět: |
Polymers
medicine.medical_treatment Cell Biomedical Engineering Brain tumor Medicine (miscellaneous) Bioengineering Photodynamic therapy 02 engineering and technology Development Conjugated system Monocytes 03 medical and health sciences Mice Drug Delivery Systems Cell Line Tumor medicine Animals General Materials Science 030304 developmental biology 0303 health sciences urogenital system Chemistry Monocyte Spheroid 021001 nanoscience & nanotechnology medicine.disease eye diseases In vitro nervous system diseases medicine.anatomical_structure Photochemotherapy Cancer research Nanoparticles Bone marrow 0210 nano-technology Glioblastoma |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1748-6963 |
| Popis: | [Aim]: To assess monocyte-based delivery of conjugated polymer nanoparticles (CPNs) for improved photodynamic therapy (PDT) in glioblastoma (GBM). [Materials and methods]: Human monocyte cells (THP-1) and murine monocytes isolated from bone marrow (mBMDMs) were employed as stealth CPN carriers to penetrate into GBM spheroids and an orthotopic model of the tumor. The success of PDT, using this cell-mediated targeting strategy, was determined by its effect on the spheroids. [Results]: CPNs did not affect monocyte viability in the absence of light and did not show nonspecific release after cell loading. Activated monocytes incorporated CPNs in a higher proportion than monocytes in their naive state, without a loss of cellular functionality. In vitro PDT efficacy using cell-mediated delivery was superior to that using non vehiculized CPNs. [Conclusion]: CPN-loaded monocytes could efficiently deliver CPNs into GBM spheroids and the orthotopic model. Improved PDT in spheroids was confirmed using this delivery strategy. |
| Databáze: | OpenAIRE |
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