The expression of angiopoietin-1 and -2 in the endometrium of women with abnormal bleeding induced by an intra-uterine device
| Autor: | H. L. Qian, HF Wang, Minfei Yang |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adult
medicine.medical_specialty Gene Expression Endometrium Biochemistry Vascular remodelling in the embryo Angiopoietin Angiopoietin-2 Immunoenzyme Techniques Internal medicine Follicular phase medicine Angiopoietin-1 Humans RNA Messenger Messenger RNA Abnormal bleeding business.industry Reverse Transcriptase Polymerase Chain Reaction Biochemistry (medical) Cell Biology General Medicine Middle Aged Prognosis Receptor TIE-2 Endocrinology medicine.anatomical_structure cardiovascular system Immunohistochemistry Female Uterine Hemorrhage business Tyrosine kinase hormones hormone substitutes and hormone antagonists Intrauterine Devices |
| Zdroj: | The Journal of international medical research. 38(1) |
| ISSN: | 0300-0605 |
| Popis: | This study identified differences in expression of angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2) in the endometrium of 33 women with abnormal bleeding induced by an intra-uterine device (IUD; 23 proliferative phase, 10 secretory phase) compared with 28 control samples from normal endometria in women without an IUD fitted (12 proliferative phase, 16 secretory phase). Expression of Ang-1, Ang-2 and endothelial cell-specific tyrosine kinase receptor-2 (Tie-2) mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR), and Ang-1 and Ang-2 protein levels were detected by immunohistochemistry. The RT-PCR results showed significantly decreased Ang-1 expression in the endometrium from IUD patients during both phases compared with the control women. Expression of Tie-2 mRNA and the Ang-1:Ang-2 mRNA ratio were also significantly decreased in endometria from IUD patients in the secretory phase compared with the control women. Immunohistochemical analysis showed elevated Ang-2 protein levels in secretory phase endometrium from IUD patients compared with the control women. These results suggest that the angiopoietin/Tie-2 system promotes vascular remodelling in the endometrium and that changes in the expression of Ang-1, Ang-2 and Tie-2 may contribute to abnormal uterine bleeding in some IUD users. |
| Databáze: | OpenAIRE |
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