Alteration of the oral microbiota may be a responsible factor, along with estrogen deficiency, by the development of larger periapical lesions
| Autor: | Priscilla Coutinho Romualdo, Léa Assed Bezerra da Silva, Raquel Assed Bezerra da Silva, Paulo Nelson-Filho, Marília Pacífico Lucisano, Magda Feres, Ana Patrícia de Sousa Pereira, Alexandra Mussolino de Queiroz |
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| Rok vydání: | 2019 |
| Předmět: |
Molar
Saliva medicine.medical_specialty medicine.drug_class Ovariectomy H&E stain 03 medical and health sciences Oral Microbiota 0302 clinical medicine Statistical significance Internal medicine medicine Animals Humans General Dentistry Mouth business.industry Microbiota Estrogens 030206 dentistry Staining Rats stomatognathic diseases Endocrinology Estrogen 030220 oncology & carcinogenesis Female Analysis of variance business |
| Zdroj: | Clinical oral investigations. 25(6) |
| ISSN: | 1436-3771 |
| Popis: | To answer the questions: (1) Does reducing estrogen levels influence the microbial composition of the oral cavity? (2) Does the presence of periapical lesion (PL) cause changes in the oral microbiota? (3) Since estrogen deficiency alters the oral microbiota, can this be one of the factors that contribute to the increase of the PL?Thirty-six rats were divided into four groups: sham (control), ovariectomy (OVX), control with PL (Sham + PL), and OVX + PL. After 9 weeks of OVX, the lower first molars were submitted to PL induction. After 21 days, the microbiological collection of the oral cavity was performed, and the animals were euthanized. The contents were evaluated by the checkerboard DNA-DNA hybridization method, to verify the prevalence of 40 bacterial species (divided into 7 microbial complexes). The blocks containing the lower first molars were submitted to histotechnical processing and staining with hematoxylin and eosin (HE), for the measurement of the periapical lesion area. The results were submitted to ANOVA and Kruskal-Wallis tests and Tukey and Dunn post-tests, with a significance level of 5%.In conditions of estrogen deficiency, there was alteration of the oral microbiota. The OVX groups had a higher amount of bacteria compared to the SHAM group in most of the microbial complexes (p0.001). The animals in the control group (with or without lesion) did not present a statistically significant difference (p0.001) in any of the microbial complexes. The PLs in OVX animals were significantly higher compared to SHAM animals (p0.001).Hypoestrogenicity conditions interfere in the oral microbiota by increasing the amount of bacteria in the saliva and influencing the progression of periapical lesions.This inedited study shows that deficiency of estrogen leads to alteration of the oral microbiota. |
| Databáze: | OpenAIRE |
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