A novel antimicrobial-containing nanocellulose scaffold for regenerative endodontics
| Autor: | Guilherme Colla, Beatriz Serrato Coelho, Maick Meneguzzo Prado, Josiane de Almeida, Lucas Soares Teixeira, Victoria Kichler, Luismar Marques Porto, Daniela Peressoni Vieira Schuldt |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
food.ingredient
Biocompatibility 02 engineering and technology Enterococcus faecalis 03 medical and health sciences 0302 clinical medicine food medicine Regenerative endodontics Agar Agar diffusion test Tissue scaffolds biology Chemistry Chlorhexidine Biofilm 030206 dentistry General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Molecular biology Streptococcus sanguinis Biofilms Actinomyces naeslundii Anti-infective agents 0210 nano-technology medicine.drug Research Article |
| Zdroj: | Restorative Dentistry & Endodontics |
| ISSN: | 2234-7666 2234-7658 |
| Popis: | Objectives The aim of this study was to evaluate bacterial nanocellulose (BNC) membranes incorporated with antimicrobial agents regarding cytotoxicity in fibroblasts of the periodontal ligament (PDLF), antimicrobial activity, and inhibition of multispecies biofilm formation. Materials and Methods The tested BNC membranes were BNC + 1% clindamycin (BNC/CLI); BNC + 0.12% chlorhexidine (BNC/CHX); BNC + nitric oxide (BNC/NO); and conventional BNC (BNC; control). After PDLF culture, the BNC membranes were positioned in the wells and maintained for 24 hours. Cell viability was then evaluated using the MTS calorimetric test. Antimicrobial activity against Enterococcus faecalis, Actinomyces naeslundii, and Streptococcus sanguinis (S. sanguinis) was evaluated using the agar diffusion test. To assess the antibiofilm activity, BNC membranes were exposed for 24 hours to the mixed culture. After sonicating the BNC membranes to remove the remaining biofilm and plating the suspension on agar, the number of colony-forming units (CFU)/mL was determined. Data were analyzed by 1-way analysis of variance and the Tukey, Kruskal-Wallis, and Dunn tests (α = 5%). Results PDLF metabolic activity after contact with BNC/CHX, BNC/CLI, and BNC/NO was 35%, 61% and 97%, respectively, compared to BNC. BNC/NO showed biocompatibility similar to that of BNC (p = 0.78). BNC/CLI showed the largest inhibition halos, and was superior to the other BNC membranes against S. sanguinis (p < 0.05). The experimental BNC membranes inhibited biofilm formation, with about a 3-fold log CFU reduction compared to BNC (p < 0.05). Conclusions BNC/NO showed excellent biocompatibility and inhibited multispecies biofilm formation, similarly to BNC/CLI and BNC/CHX. |
| Databáze: | OpenAIRE |
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