Protective effects of sea cucumber (Holothuria atra) extract on testicular dysfunction induced by immune suppressant drugs in Wistar rats
| Autor: | Gehan B A Youssef, Dalia Saad, Mohamed Mohamed Soliman, Alaa A. Mohamed |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine Male Urology Pharmacology medicine.disease_cause Testicular Diseases 03 medical and health sciences Interferon-gamma 0302 clinical medicine Endocrinology Immune system medicine Animals Holothuria Testosterone Rats Wistar skin and connective tissue diseases Cytotoxicity Testicular Hormones Kidney biology business.industry Interleukin-6 Plant Extracts Superoxide Dismutase Tumor Necrosis Factor-alpha General Medicine Luteinizing Hormone Catalase Rats Oxidative Stress 030104 developmental biology medicine.anatomical_structure Methotrexate 030220 oncology & carcinogenesis biology.protein Follicle Stimulating Hormone business Oxidative stress Biomarkers Immunosuppressive Agents medicine.drug |
| Zdroj: | Andrologia. 50(6) |
| ISSN: | 1439-0272 |
| Popis: | The current study was aimed to evaluate the protective effect of Holothurian atra (HA) extract; naturally occurring marine resource, against methotrexate (MTX) induced testicular dysfunction. Mature rats received either MTX (20 mg/kg, intraperitoneally) or saline on the 7th day of experiment al design. Seven days prior and after MTX-injection, rats received HA at dose of 300 mg/kg intragastrically (HA + MTX group; HA group alone). Serum was extracted and testicular tissues were examined for the changes in serum biochemistry (liver & kidney biomarkers, testicular hormones and antioxidants), molecular and histopthological alterations using RT-PCR and immunohistochemistry. MTX-injected rats induced alteration in all testicular parameters. Prior administration of HA ameliorated the MTX-induced oxidative stress. HA administration normalised MTX-induced decrease in serum levels of interleukin-6 (IL-6), tumour necrosis factor alpha (TNF-α), interferon-gamma (IFN-γ), reproductive hormones (FSH, LH and testosterone) and antioxidants GST, SOD and catalase. MTX-injected rats down-regulated mRNA expression of GST, SOD, steroidogenesis associated genes, IFN-γ, Bcl2 and NFKB. MTX up-regulated BAX expression and caspase 9 immunoreactivity that were ameliorated in HA + MTX group. Collectively, HA ameliorated and restored all altered genes. In conclusion, HA is a promising supplement that is helpful in protection against testicular cytotoxicity and dysfunction induced by methotrexate. |
| Databáze: | OpenAIRE |
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