Isolation of Breast cancer CTCs with multitargeted buoyant immunomicrobubbles
| Autor: | Guixin Shi, Santosh Kesari, Anthony D. Elias, Sandeep C. Pingle, Guankui Wang, Michael Benchimol, Yu-Tsueng Liu, Yasan Yeh, Dmitri Simberg, Jessica F. Jones, Halli Benasutti, Li-En Hsieh |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
animal structures Cell Breast Neoplasms Cell Separation Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Colloid and Surface Chemistry Breast cancer Circulating tumor cell Cell Line Tumor Biomarkers Tumor medicine Humans Physical and Theoretical Chemistry Microbubbles biology Chemistry Cancer Epithelial cell adhesion molecule Surfaces and Interfaces General Medicine Epithelial Cell Adhesion Molecule Neoplastic Cells Circulating medicine.disease Metastatic breast cancer 030104 developmental biology medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis Immunology MCF-7 Cells biology.protein Cancer research Keratins Female Antibody Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 161:200-209 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2017.10.060 |
| Popis: | Circulating tumor cells (CTCs) are extremely rare cells found in blood of metastatic cancer patients. There is a need for inexpensive technologies for fast enrichment of CTCs from large blood volumes. Previous data showed that antibody-conjugated lipid shell immuno-microbubbles (MBs) bind and isolate cells from biological fluids by flotation. Here, blood-stable MBs targeted to several surface markers for isolation of breast tumor cells were developed. MBs coated with anti-human EpCAM antibodies showed efficient binding of EpCAM+ breast cancer cell lines SKBR-3, MCF-7, and MDA-MB-453, whereas anti-human EGFR MBs showed binding of EpCAMLOW/NEGATIVE cell lines MDA-MB-231 and BT-549. Multitargeted anti-human EpCAM/EGFR MBs bound all cell lines with over 95% efficiency. Highly concentrated MB-bound tumor cells were collected in a microliter volume via an inverted vacuum-assisted harvesting setup. Using anti-EpCAM and/or anti-EpCAM/EGFR MBs, an efficient (70–90%) recovery and fast (30 min) isolation of the above-mentioned cells and cell clusters was achieved from 7.5 mL of spiked human blood. Using anti-EpCAM MBs and anti-EpCAM/EGFR MBs, cytokeratin-positive, CD45-negative CTCs were detected in 62.5% (10/16) of patients with metastatic breast cancer and CTC clusters were detected in 41.7% (5/12) of CTC-positive samples. Moreover, in some samples MBs isolated cytokeratin positive, CD45 negative tumor-derived microparticles. None of these structures were detected in blood from non-epithelial malignancies. The fast and inexpensive multitargeted platform for batch isolation of CTCs can promote research and clinical applications involving primary tumors and metastases. |
| Databáze: | OpenAIRE |
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