A Polarity‐Sensitive Ratiometric Fluorescence Probe for Monitoring Changes in Lipid Droplets and Nucleus during Ferroptosis
| Autor: | Qian Cao, Duo Mao, Cai-Ping Tan, Bin Liu, Shidang Xu, Zong-Wan Mao, Liu-Yi Liu, Kangnan Wang |
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| Rok vydání: | 2021 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Polarity (physics) 010402 general chemistry 01 natural sciences Catalysis Lipid droplet medicine Ferroptosis Humans Fluorescent Dyes Cell Nucleus chemistry.chemical_classification Reactive oxygen species Molecular Structure Lipid peroxide 010405 organic chemistry Chemistry Lipid Droplets General Medicine General Chemistry Fluorescence Ratiometric fluorescence 0104 chemical sciences medicine.anatomical_structure Cytoplasm Biophysics Reactive Oxygen Species Nucleus |
| Zdroj: | Angewandte Chemie International Edition. 60:15095-15100 |
| ISSN: | 1521-3773 1433-7851 |
| Popis: | Ferroptosis regulates cell death through reactive oxygen species (ROS)-associated lipid peroxide accumulation, which is expected to affect the structure and polarity of lipid droplets (LDs), but with no clear evidence. Herein, we report the first example of an LD/nucleus dual-targeted ratiometric fluorescent probe, CQPP, for monitoring polarity changes in the cellular microenvironment. Due to the donor-acceptor structure of CQPP, it offers ratiometric fluorescence emission and fluorescence lifetime signals that reflect polarity variations. Using nucleus imaging as a reference, CQPP was applied to report the increase in LD polarity and the homogenization of polarity between LDs and cytoplasm in the ferroptosis model. This LD/nucleus dual-targeted fluorescent probe shows the great potential of using fluorescence imaging to study ferroptosis and ferroptosis-related diseases. |
| Databáze: | OpenAIRE |
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