Protein Kinases in Mitochondria of the InvertebrateArtemia franciscana
| Autor: | Ana M. Seguido, Margarita Fernández-Renart, Carmen G. Vallejo |
|---|---|
| Přispěvatelé: | Dirección General de Investigación Científica y Técnica, DGICT (España), Ministerio de Educación y Ciencia (España) |
| Rok vydání: | 1997 |
| Předmět: |
Phosphopeptides
Biophysics Centrifugation Isopycnic Protein Serine-Threonine Kinases Biology Mitogen-activated protein kinase kinase Biochemistry Cytosol Microsomes Casein kinase 2 alpha 1 Animals c-Raf Casein Kinase II Protein kinase A Molecular Biology MAPK14 cAMP-dependent protein kinase Heparin Cyclin-dependent kinase 2 Cyclic AMP-Dependent Protein Kinases Casein kinase II Mitochondria Cell biology Kinetics biology.protein Casein kinase 1 Artemia Casein kinase 2 |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0003-9861 |
| DOI: | 10.1006/abbi.1996.9842 |
| Popis: | The information concerning protein kinases in animal mitochondria is scarce and related only to mammals. No data are available for invertebrates. We demonstrate here the presence of casein kinase II (CK II) and cAMP-dependent protein kinase (PKA) in the purified mitochondria of the crustaceanArtemia franciscana.Whereas the mitochondrial CK II showed the same characteristics of the cytosolic enzyme, mitochondrial PKA had an apparentKmfor its substrate Kemptide 1 order of magnitude lower than that of the cytosolic enzyme. CK II and PKA phosphorylate different sets of proteins inArtemiamitochondriain vitro.The use of an activity gel assay has allowed the detection of additional protein kinases, as yet unidentified, inArtemiamitochondria. This work was supported by Grants PB 92-0059 and PB 92-0774 from the Dirección General de Investigación Científica y Técnica (Ministerio de Educación y Ciencia, Spain). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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