Oocyte origin affects the in vitro embryo production and development of Holstein (Bos taurus taurus) - Gyr (Bos taurus indicus) reciprocal cross embryos
| Autor: | Célio de Freitas, R. V. Serapião, A. J. R. Camargo, L. T. Iguma, L. Z. Oliveira, Rui da Silva Verneque, Bruno Campos de Carvalho, Clara Slade Oliveira, Luiz Sérgio de Almeida Camargo |
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| Rok vydání: | 2019 |
| Předmět: |
Male
animal structures animal diseases Embryonic Development Mothers Oocyte Retrieval Semen Fertilization in Vitro Biology Embryo Culture Techniques Andrology 03 medical and health sciences fluids and secretions 0302 clinical medicine Endocrinology Human fertilization Food Animals Pregnancy medicine Animals Cells Cultured reproductive and urinary physiology 030219 obstetrics & reproductive medicine 0402 animal and dairy science food and beverages Embryo 04 agricultural and veterinary sciences General Medicine Embryo Mammalian medicine.disease Oocyte 040201 dairy & animal science Sperm Breed Blastocyst Treatment Outcome medicine.anatomical_structure Oocytes Hybridization Genetic Gestation Cattle Female Animal Science and Zoology Estrus Synchronization |
| Zdroj: | Animal Reproduction Science. 209:106165 |
| ISSN: | 0378-4320 |
| DOI: | 10.1016/j.anireprosci.2019.106165 |
| Popis: | A reciprocal crossbred embryo production approach was used to assess effects of maternal breed on embryo development in tropical conditions (average temperature 22.0 °C and 77.9% relative humidity). Oocytes were recovered by ovum pick-up (OPU) from Gyr and Holstein donors (n = 90 Holstein and 83 Gyr OPUs). Female F1 embryos were produced by fertilization with sperm bearing X-chromosomes from Holstein semen (n = 615 Gyr oocytes) or Gyr semen (n = 255 Holstein oocytes). Blastocysts were transferred to recipients 168 h post-insemination (h.p.i.) (n = 70–144) and there were assessments of pregnancies until birth. Oocyte number per OPU (Gyr 10.0 ± 0.7 compared with Holstein 6.3 ± 0.4) and percentage viable oocytes (Gyr 78.8 ± 1.9% compared with Holstein 71.2 ± 2.2%) were less for Holstein donor animals. There was a 2.8 fold fewer total number of F1 blastocysts when Holstein donors were used (Gyr: 260, Holstein: 91). Pregnancy assessment during the different stages of gestation indicated the percentage pregnancy was less when embryos were produced from Holstein oocytes (Gyr and Holstein respectively: early pregnancy, 47.9% compared with 38.6%; mid-pregnancy, 44.4% compared with 31.4%; late pregnancy, 41.0% compared with 22.9%). Pregnancy length was also affected by maternal breed (Gyr: 280.8 ± 0.6, Holstein: 286.3 ± 0.7). It is concluded that in a tropical environment the maternal breed affects crossbred embryo development with pregnancy rates during the latter stages of gestation being greater when Gyr oocytes are used for production of embryos. |
| Databáze: | OpenAIRE |
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