Monoamines Inhibit GABAergic Neurons in Ventrolateral Preoptic Area That Make Direct Synaptic Connections to Hypothalamic Arousal Neurons
| Autor: | Yuchio Yanagawa, Yuki Saito, Takashi Maejima, Mitsuhiro Nishitani, Michihiro Mieda, Takeshi Sakurai, Emi Hasegawa |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Serotonin Mice Transgenic Biology Mice Norepinephrine 03 medical and health sciences 0302 clinical medicine Neural Pathways medicine Animals GABAergic Neurons Research Articles General Neuroscience Preoptic Area Orexin Preoptic area Electrophysiology 030104 developmental biology medicine.anatomical_structure Monoamine neurotransmitter nervous system Hypothalamus Hypothalamic Area Lateral GABAergic Neuron Arousal Sleep Tuberomammillary nucleus Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | The Journal of Neuroscience. 38:6366-6378 |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.2835-17.2018 |
| Popis: | The hypothalamus plays an important role in the regulation of sleep/wakefulness states. While the ventrolateral preoptic nucleus (VLPO) plays a critical role in the initiation and maintenance of sleep, the lateral posterior part of the hypothalamus contains neuronal populations implicated in maintenance of arousal, including orexin-producing neurons (orexin neurons) in the lateral hypothalamic area (LHA) and histaminergic neurons in the tuberomammillary nucleus (TMN). During a search for neurons that make direct synaptic contact with histidine decarboxylase-positive (HDC+), histaminergic neurons (HDC neurons) in the TMN and orexin neurons in the LHA of male mice, we found that these arousal-related neurons are heavily innervated by GABAergic neurons in the preoptic area including the VLPO. We further characterized GABAergic neurons electrophysiologically in the VLPO (GABA(VLPO) neurons) that make direct synaptic contact with these hypothalamic arousal-related neurons. These neurons (GABA(VLPO→HDC) or GABA(VLPO→orexin) neurons) were both potently inhibited by noradrenaline and serotonin, showing typical electrophysiological characteristics of sleep-promoting neurons in the VLPO. This work provides direct evidence of monosynaptic connectivity between GABA(VLPO) neurons and hypothalamic arousal neurons and identifies the effects of monoamines on these neuronal pathways. SIGNIFICANCE STATEMENT Rabies-virus-mediated tracing of input neurons of two hypothalamic arousal-related neuron populations, histaminergic and orexinergic neurons, showed that they receive similar distributions of input neurons in a variety of brain areas, with rich innervation by GABAergic neurons in the preoptic area, including the ventrolateral preoptic area (VLPO), a region known to play an important role in the initiation and maintenance of sleep. Electrophysiological experiments found that GABAergic neurons in the VLPO (GABA(VLPO) neurons) that make direct input to orexin or histaminergic neurons are potently inhibited by noradrenaline and serotonin, suggesting that these monoamines disinhibit histamine and orexin neurons. This work demonstrated functional and structural interactions between GABA(VLPO) neurons and hypothalamic arousal-related neurons. |
| Databáze: | OpenAIRE |
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