Flow cytometric investigation of peri-anaesthetic anaphylaxis using CD63 and CD203c
| Autor: | Judith Elizabeth Hall, Potteth Sudheer, A. W. Rowbottom, Paul Williams, G. F. Read |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Drug Allergy media_common.quotation_subject Platelet Membrane Glycoproteins Basophil Histamine Release Sensitivity and Specificity Drug Hypersensitivity chemistry.chemical_compound Antigens CD Immunopathology Humans Medicine Anesthesia Pyrophosphatases Intraoperative Complications Anaphylaxis Anesthetics media_common CD63 Phosphoric Diester Hydrolases Tetraspanin 30 business.industry Flow Cytometry medicine.disease Basophils Basophil activation Anesthesiology and Pain Medicine medicine.anatomical_structure chemistry Immunology Female Neuromuscular Blocking Agents business Biomarkers Histamine |
| Zdroj: | Anaesthesia. 60:251-256 |
| ISSN: | 1365-2044 0003-2409 |
| DOI: | 10.1111/j.1365-2044.2004.04086.x |
| Popis: | The investigation of anaphylactic reactions in the peri-operative period is difficult. Elevation of serum tryptase levels is a good indicator of an anaphylactic event but the ability of subsequent investigations to identify the drug(s) responsible for the reaction is still potentially unreliable. The aim of this study was to examine basophil activation as an investigative tool. We performed flow cytometric analysis of the expression on the cell surface of the basophil activation markers CD63 and CD203c and measured histamine release in 21 patients who were referred with possible peri-operative anaphylaxis. The sensitivity of CD63, CD203c, basophil histamine release and skin prick for the muscle relaxants was found to be 79%, 36%, 36% and 64%, respectively; the specificity was found to be 100%. These results demonstrate the difficulty in investigating the cause of an unexpected clinical event following drug administration, but the higher sensitivity of neo-expression on the cell surface of CD63 suggests that flow cytometric analysis of its neo-expression on basophils in vitro may be a diagnostic aid. |
| Databáze: | OpenAIRE |
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