Microbial volatile compounds-induced cytotoxicity in the yeast Saccharomyces cerevisiae: The role of MAPK signaling and proteasome regulatory pathway
| Autor: | Yueh Lin Ho, Pei Hsuan Wu, Ching Chuan Liu, Ching Han Chang, Tzong Shiann Ho, Je Chiuan Ye, Ching Han Wang, Huang Mo Sung, Hao Jen Huang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Transcriptional Activation
Cytoplasm Proteasome Endopeptidase Complex Saccharomyces cerevisiae Proteins Environmental Engineering MAP Kinase Signaling System Health Toxicology and Mutagenesis 0208 environmental biotechnology Saccharomyces cerevisiae 02 engineering and technology 010501 environmental sciences 01 natural sciences Downregulation and upregulation Cell Wall Air Pollution Environmental Chemistry 0105 earth and related environmental sciences Air Pollutants Volatile Organic Compounds biology Chemistry Public Health Environmental and Occupational Health Enterobacter aerogenes General Medicine General Chemistry biology.organism_classification Pollution Yeast 020801 environmental engineering Cell biology Proteasome Proteasome assembly Chaperone (protein) Mitogen-activated protein kinase biology.protein Mitogen-Activated Protein Kinases Regulatory Pathway Reactive Oxygen Species Molecular Chaperones |
| Zdroj: | Chemosphere. 233:786-795 |
| ISSN: | 0045-6535 |
| Popis: | Microbial volatile organic compounds (mVCs) are formed in the metabolism of microorganisms and widely distributed in nature and pose threats to human health. However, the air pollution by microorganisms is a situation which is poorly understood. In this study, the cytotoxicity of E. aerogenes VCs was evaluated in the model organism Saccharomyces cerevisiae. E. aerogenes VCs inhibited the survival of yeast and triggered the formation of intracellular reactive oxygen species (ROS). The hypersensitive of MAP kinase mpk1/slt2 and 19S regulatory assembly chaperone adc17 mutants to the E. aerogenes VCs indicated cell wall integrity (CWI) pathway together with stress-inducible proteasome assembly regulation are essentially involved in mVCs tolerance mechanism. Furthermore, exposure to the mVCs resulted in the transcriptional upregulation of the CWI pathway, the regulatory particle assembly chaperones, and genes involved in proteasome regulations. Our research suggested that the ROS/MAPK signaling and proteasome regulatory pathway play pivotal roles in the integration and fine-tuning of the mVCs stress response. This study provides a molecular framework for future study of the effects of mVCs on more complex organisms, such as humans. |
| Databáze: | OpenAIRE |
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