Cystic Fibrosis Airway Epithelia Fail to Kill Bacteria Because of Abnormal Airway Surface Fluid
| Autor: | Michael J. Welsh, Sue M. Travis, Jeffrey J. Smith, E. Peter Greenberg |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Cystic Fibrosis
Bacterial killing Sodium Chloride Biology Cystic fibrosis Epithelium General Biochemistry Genetics and Molecular Biology Microbiology 03 medical and health sciences 0302 clinical medicine Bacteriocins Chlorides Chloride Channels medicine Humans Lung 030304 developmental biology 0303 health sciences Dose-Response Relationship Drug Biochemistry Genetics and Molecular Biology(all) Biological Transport respiratory system biology.organism_classification medicine.disease respiratory tract diseases 3. Good health Ambient air Dose–response relationship 030228 respiratory system Lung disease Pseudomonas aeruginosa Immunology Microscopy Electron Scanning Extracellular Space Airway Bacteria |
| Zdroj: | Cell. 85:229-236 |
| ISSN: | 0092-8674 |
| Popis: | thelia causes chronic bacterial infections in cystic fi- al. (1989) have shown that airway surface fluid from brosis (CF) airways. Here, we show that common CF patients with CF has increased concentrations of Cl2 pathogens were killed when added to the apical sur- and Na 1 when compared with that of normal subjects. face of normal airway epithelia. In contrast, these bac- But how do these abnormalities relate to airway infecteria multiplied on CF epithelia. We found that bacteri- tions? cidal activity was present in airway surface fluid of both normal and CF epithelia. However, because bacterial killing required a low NaCl concentration and Results because CF surface fluid has a high NaCl concentration, CF epithelia failed to kill bacteria. This defect was Normal but Not CF Epithelia Kill Bacteria corrected by reducing the NaCl concentration on CF Applied to the Apical Surface epithelia. These data explain how the loss of CFTR Cl 2 Human airways are continually exposed to bacteria in channels may lead to lung disease and suggest new ambient air (10 3 /m 3 ) and to aspirated bacteria (DeKoster approaches to therapy. |
| Databáze: | OpenAIRE |
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