Cytotoxic effect of endodontic irrigants in vitro
Autor: | Sevda Muftuoglu, Donika Bajrami, Veton Hoxha, Selen Küçükkaya, Ömer Görduysus, Nacije Dilara Zeybek |
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Rok vydání: | 2014 |
Předmět: |
medicine.medical_specialty
Serial dilution Cell Survival Periodontal Ligament Dentistry Endodontics Root canal irrigant medicine Periodontal fiber Cytotoxic T cell Animals Viability assay Sodium Hypochlorite - toxicity Cytotoxicity Cell Death Root Canal Irrigants business.industry Chemistry General Medicine Molecular biology In vitro Rats Chlorhexidine - toxicity business In Vitro Studies |
Zdroj: | Medical Science Monitor Basic Research |
ISSN: | 2325-4416 |
Popis: | Background Cytotoxicity of root canal irrigants is important due to their close contact with host tissues. The aim of this study was to assess the cytotoxic effect of NaOCl 3%, Chx 2%, and MTAD on rat periodontal ligament fibroblasts, at 0.1 and 100 µl/mL, using WST-1 colorimetric method. Material and methods Rat ligamental fibroblasts were exposed to the irrigants and their viability was assessed after 1, 24, 48, and 72 h. The measurements were determined using WST-1 assay, using a micro ELISA reader. Results At 100 ml/L all 3 irrigants were strongly cytotoxic, although CHX was less so than NaOCl and MTAD. At the 0.1 ml/L concentration, NaOCl and MTAD were only moderately cytotoxic, whereas Chx was highly deleterious to cell viability at all time points. There was a significant influence of the dilution rate of the substance, because the odds ratio for cell viability being over 50% was increased 51 times between the 100 ml/L and 0.1 ml/L dilutions. Conclusions It seems that irrigating solutions should be used at lower concentrations to enhance cell viability. |
Databáze: | OpenAIRE |
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