Infection with a virus generates a polyclonal immune response with broad alloreactive potential
| Autor: | Xiaoqian Zhang, Paula P.M.C. van Miert, Ellen M.W. van der Meer-Prins, Frans H.J. Claas, Heleen van den Heuvel, Jacqueline D.H. Anholts |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Epstein-Barr Virus Infections Herpesvirus 4 Human Isoantigens Immunology Cytomegalovirus Human leukocyte antigen CD8-Positive T-Lymphocytes Virus 03 medical and health sciences 0302 clinical medicine Immune system HLA Antigens Virus-specific T cells Immunology and Allergy Humans TCR cross-reactivity Antigens Viral Alloreactivity Cells Cultured Polymorphism Genetic biology Repertoire General Medicine Transplantation HLA 030104 developmental biology Polyclonal antibodies Healthy individuals Cytomegalovirus Infections Polyclonal immune response biology.protein Female Peptides Immunologic Memory CD8 030215 immunology |
| Zdroj: | Human Immunology, 80(2), 97-102. ELSEVIER SCIENCE INC |
| Popis: | Virus-specific T cells have been shown to cross-react with allogeneic HLA (allo-HLA) at a clonal level. However, the impact of a single virus on the allorepertoire has never been investigated at the polyclonal level.We made an inventory of the incidence and specificity of allo-HLA-cross-reactive-virus-specific CD8(+) T cells in 24 healthy individuals. T cells were stained for 25 virus-specific tetramers, and mixed-lymphocyte reactions were performed against a panel of HLA-typed allostimulators. Allospecificity was confirmed by IFN gamma-ELISA using T-cell clones against a panel of HLA-typed cell-lines.The polyclonal immune repertoire directed against CMV alone was associated with a memory response against six allo-HLA molecules. Besides, a single allostimulator activated memory T-cell responses with multiple viral specificities.Concluding, a single virus can substantially broaden the allo-HLA memory T-cell repertoire. This study only looked at CMV- and EBV-specific T cells, whereas the immune repertoire consists of T cells directed against many different viruses. Hence, transplant patients receiving an HLA-mismatched graft may already express a poly clonal repertoire of anti-donor-memory T cells before transplantation. |
| Databáze: | OpenAIRE |
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