Coralline shape of the bacterial nucleoid after cryofixation
| Autor: | R. Johansen, W. Villiger, Bernd Bohrmann, E. Kellenberger |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
DNA
Bacterial Bacterial nucleoid Biology medicine.disease_cause Microbiology Immunohistochemistry Chromatin Cell biology Cryofixation Fixatives Microscopy Electron Cytoplasm Microscopy medicine Ultrastructure Escherichia coli Immunologic Techniques Nucleoid Molecular Biology Immunostaining Research Article |
| Zdroj: | Journal of bacteriology. 173(10) |
| ISSN: | 0021-9193 |
| Popis: | A new procedure of immunostaining sections of cryofixed and freeze-substituted Escherichia coli shows that DNA extends from its bulk into small ribosome-free spaces throughout the cytoplasm, resulting in a coralline-shaped nucleoid. Low-resolution imaging of a bacterium reconstructed from serial sections demonstrated that the small excrescencies are not resolved. The resulting photograph shows the same features as phase-contrast light micrographs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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