Molecular epidemiology of hospital-onset methicillin-resistant Staphylococcus aureus infections in Southern Chile
| Autor: | Heriberto Fernández, Gustavo Medina, Claudia Sola, Carola Otth, Ana L. Egea, José Luis Bocco, Laura Otth, M. Wilson |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Methicillin-Resistant Staphylococcus aureus Microbiology (medical) medicine.medical_specialty CIENCIAS MÉDICAS Y DE LA SALUD Adolescent Ciencias de la Salud MRSA Biology Healthcare-onset infections medicine.disease_cause Microbiology Young Adult Medical microbiology Prevalence medicine Pulsed-field gel electrophoresis Humans Chile Child Aged Cross Infection Molecular Epidemiology Molecular epidemiology SCCmec Infant Ética Médica General Medicine Middle Aged Staphylococcal Infections biochemical phenomena metabolism and nutrition bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus Subtyping Infectious Diseases Staphylococcus aureus Child Preschool Multilocus sequence typing Female |
| Zdroj: | European Journal of Clinical Microbiology & Infectious Diseases. 32:1533-1540 |
| ISSN: | 1435-4373 0934-9723 |
| Popis: | Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen of public health importance. In Chile, the Cordobes/Chilean clone was the predominant healthcareassociated MRSA (HA-MRSA) clone in 1998. Since then, the molecular epidemiological surveillance of MRSA has not been performed in Southern Chile. We aimed to investigate the molecular epidemiology of HA-MRSA infections in Southern Chile to identify the MRSA clones involved, and their evolutionary relationships with epidemic international MRSA lineages. A total of 303 single inpatient isolates of S. aureus were collected in the Valdivia County Hospital (2007–2008), revealing 33 % (100 MRSA/303) prevalence for HA-MRSA infections. The SCCmec types I and IV were identified in 97 % and 3 % of HA-MRSA, respectively. All isolates lacked the pvl genes. A random sample (n = 29) of all MRSA was studied by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), SCCmec subtyping, agr and spa typing, and virulence genes profiling. PFGE analysis revealed the predominance (89 %, 26/29) of pulsotype A and three additional pulsotypes, designated H1, I33, and G1. Pulsotype A (ST5-SCCmecI-spa-t149) is clonally related to the Cordobes/Chilean clone. Pulsotype H1 (ST5-SCC mecIVNT-spa-t002) is genetically related to the Pediatric clone (ST5-SCCmecIV). Pulsotype I33 (ST5-SCCmecIVc-spa-t002) is clonally related by PFGE to the community-associated MRSA (CA-MRSA) clone spread in Argentina, I-ST5-IVa-PVL+ . The G1 pulsotype (ST8-SCCmecIVc-spa-t024) is clonally related to the epidemic USA300 CA-MRSA. Here, we demonstrate the stability of the Cordobes/Chilean clone over time as the major HA-MRSA clone in Southern Chile. The identification of two CA-MRSA clones might suggest that these clones have entered into the healthcare setting from the community. These results emphasize the importance of the local surveillance of MRSA infections in the community and hospital settings. Fil: Medina, G.. Universidad Austral de Chile. Facultad de Medicina. Instituto de Microbiología Clínica; Chile Fil: Egea, Ana Lía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Otth, C.. Universidad Austral de Chile. Facultad de Medicina. Instituto de Microbiología Clínica; Chile Fil: Otth, L.. Universidad Austral de Chile. Facultad de Medicina. Instituto de Microbiología Clínica; Chile Fil: Fernández, H.. Universidad Austral de Chile. Facultad de Medicina. Instituto de Microbiología Clínica; Chile Fil: Bocco, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Wilson, M.. Universidad Austral de Chile. Facultad de Medicina. Instituto de Microbiología Clínica; Chile Fil: Sola, Claudia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina |
| Databáze: | OpenAIRE |
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