Growth Factor Regulation of Interleukin-1β-Induced Nitric Oxide Synthase and GTP: Cyclohydrolase Expression in Cultured Smooth Muscle Cells
| Autor: | Timothy Scott-Burden, Tong Ge, Paul M. Vanhoutte, E. Elizondo, C. M. Boulanger |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Vascular smooth muscle
GTP' GTP cyclohydrolase I medicine.medical_treatment Biophysics Gene Expression Fibroblast growth factor Biochemistry Muscle Smooth Vascular Transforming Growth Factor beta medicine Animals RNA Messenger GTP Cyclohydrolase Growth Substances Molecular Biology Aorta Cells Cultured Platelet-Derived Growth Factor biology Growth factor Cell Biology Tetrahydrobiopterin Blotting Northern Rats Cell biology Nitric oxide synthase Kinetics Cell culture biology.protein Amino Acid Oxidoreductases Nitric Oxide Synthase Interleukin-1 medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 196:1261-1266 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1993.2388 |
| Popis: | Induction of NO synthase expression by interleukin-1 beta in cultured vascular smooth muscle cells from rat aortas was accompanied by simultaneous induction of GTP: cyclohydrolase I. This enzyme regulates the de novo synthesis pathway for tetrahydrobiopterin, an essential cofactor for the catalytic conversion of L-arginine to L-citrulline and NO by inducible NO synthase. Inhibition of GTP: cyclohydrolase attenuated NO production by interleukin-1 beta-stimulated smooth muscle cells. Peptide growth factors such as fibroblast growth factor, platelet-derived growth factor and transforming growth factor beta 1 and the protease thrombin have been shown to modulate the production NO by cytokine-treated smooth muscle cells. These peptide agonists also regulated the induction of NO synthase and GTP: cyclohydrolase mRNA expression. |
| Databáze: | OpenAIRE |
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