The lncRNAs involved in mouse airway allergic inflammation following induced pluripotent stem cell-mesenchymal stem cell treatment

Autor: Xing-Liang Fan, Qiu-Ning Yu, Cheng-Lin Li, Yueqi Sun, Meng-Xia Deng, Jianbo Shi, Qing-Ling Fu, Shu-Yue Wang, Wenxiang Gao
Jazyk: angličtina
Předmět:
0301 basic medicine
Induced pluripotent stem cells-mesenchymal stem cells (iPSC-MSCs)
Induced Pluripotent Stem Cells
lncRNAs
Medicine (miscellaneous)
Inflammation
Biology
Microarray
Mesenchymal Stem Cell Transplantation
Real-Time Polymerase Chain Reaction
Biochemistry
Genetics and Molecular Biology (miscellaneous)

Allergic inflammation
Proinflammatory cytokine
Immunomodulation
03 medical and health sciences
Th2 Cells
medicine
Hypersensitivity
Animals
RNA
Messenger

Induced pluripotent stem cell
Lung
Induced stem cells
Mice
Inbred BALB C

Research
Gene Expression Profiling
Mesenchymal stem cell
Mesenchymal Stem Cells
Cell Biology
Asthma
030104 developmental biology
Gene Ontology
Gene Expression Regulation
Immunology
Cancer research
Molecular Medicine
Cytokines
Female
RNA
Long Noncoding

Stem cell
medicine.symptom
Adult stem cell
Zdroj: Stem Cell Research & Therapy
ISSN: 1757-6512
DOI: 10.1186/s13287-016-0456-3
Popis: Background We have previously reported that induced pluripotent stem cell (iPSC)-mesenchymal stem cells (MSCs) alleviated asthma inflammation in mice. Long noncoding RNAs (lncRNAs) were recently reported as being involved in the immune responses. However, whether lncRNAs are associated with iPSC-MSC immunomodulation in allergic inflammation is still unclear. Methods Mice were induced into an asthmatic state and received treatment consisting of iPSC-MSCs. Memory T cells isolated from sensitized mice were challenged and co-cultured with iPSC-MSCs in vitro. Total RNA from the lungs and separated T cells were processed with an lncRNA/mRNA microarray. A series of bioinformatics technologies were used to screen the target lncRNAs. Results iPSC-MSCs significantly prevented asthma inflammation and decreased the Th2 cytokine levels. Over 1300 lncRNAs were differentially expressed after the induction of asthma, and 846 or 4176 lncRNAs were differentially expressed with iPSC-MSC treatment in mice or in vitro, respectively. After overlapping the differentially expressed lncRNAs produced in a similar manner in mice and in vitro, 23 lncRNAs and 96 mRNAs were selected, in which 58 protein-coding genes were predicted to be potential targets of the 23 lncRNAs. Furthermore, using a series of bioinformatics technologies, 9 lncRNAs co-expressed with the most differentially expressed mRNAs, which were enriched in terms of the immune response, were screened out via Pearson’s correlation coefficient with mRNAs that were involved with inflammatory cytokines and receptors. lncRNAs MM9LINCRNAEXON12105+ and AK089315 were finally emphasized via quantitative real-time PCR validation. Conclusions Our results suggested that aberrant lncRNA profiles were present after asthma induction and iPSC-MSC treatment, suggesting potential targets of allergic inflammation and iPSC-MSC-mediated immunomodulation. Electronic supplementary material The online version of this article (doi:10.1186/s13287-016-0456-3) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE