Reexamining the role of the accessory plasmid pAtC58 in the virulence of Agrobacterium tumefaciens strain C58
| Autor: | Andrew N. Binns, Zhenying Liu, Gauri R. Nair |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Transcriptional Activation
Rhizobiaceae Physiology Virulence Plant Science Microbiology Plasmid Transformation Genetic Plant Tumors Gene expression Genetics Plant Tumor-Inducing Plasmids Gene Regulation of gene expression biology Agrobacterium tumefaciens Gene Expression Regulation Bacterial Plants biology.organism_classification Plants Genetically Modified Transformation (genetics) Focus Issue: The Agrobacterium-Plant Cell Interaction Mutation |
| Zdroj: | Plant physiology. 133(3) |
| ISSN: | 0032-0889 |
| Popis: | Isogenic strains of Agrobacterium tumefaciens carrying pTiC58, pAtC58, or both were constructed and assayed semiquantitatively and quantitatively for virulence and vir gene expression to study the effect of the large 542-kb accessory plasmid, pAtC58, on virulence. Earlier studies indicate that the att (attachment) genes of A. tumefaciens are crucial in the ability of this soil phytopathogen to infect susceptible host plants. Mutations in many att genes, notably attR and attD, rendered the strain avirulent. These genes are located on pAtC58. Previous work also has shown that derivatives of the wild-type strain C58 cured of pAtC58 are virulent as determined by qualitative virulence assays and, hence, pAtC58 was described as nonessential for virulence. We show here that the absence of pAtC58 in pTiC58-containing strains results in reduced virulence but that disruption of the attR gene does not result in avirulence or a reduction in virulence. Our studies indicate that pAtC58 has a positive effect on vir gene induction as revealed by immunoblot analysis of Vir proteins and expression of a PvirB::lacZ fusion. |
| Databáze: | OpenAIRE |
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