Splenic function and red blood cell deformability: The beneficial effects of spleen autotransplantation in animal experiments
| Autor: | Györgyi Szabó, Istvan Furka, Sándor Imre, Ferenc Kiss, Katalin Peto, Erika Sajtos, Norbert Nemeth, Andrea Furka, Iren Miko, Endre Brath |
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| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Splenic function Physiology medicine.medical_treatment Splenectomy Urology Spleen Beagle Dogs Erythrocyte Deformability Physiology (medical) medicine Animals Erythrocyte deformability Elméleti orvostudományok Beneficial effects business.industry Orvostudományok Hematology Autotransplantation Surgery Red blood cell medicine.anatomical_structure Hemorheology Cardiology and Cardiovascular Medicine business Follow-Up Studies |
| Zdroj: | Clinical Hemorheology and Microcirculation. 45:281-288 |
| ISSN: | 1386-0291 |
| DOI: | 10.3233/ch-2010-1307 |
| Popis: | Erythrocyte deformability could be a suitable parameter reflecting hyposplenic-asplenic conditions after splenectomy or spleen autotransplantation. The aim of this study was to analyze the efficacy of spleen autotransplantation using filtrometry and ektacytometry for testing erythrocyte deformability, besides complex structural, functional, hematological, hemostaseological and immunological examinations. Healthy beagle dogs were subjected to the study in the following experimental groups: "Sham"-sham operated control, "SE"-splenectomy, "AU-5" and "AU-10"-autotransplantation with 5 or 10 spleen chips using "Furka's spleen chip" technique after splenectomy. Blood samples were taken before the operations and monthly afterwards for 1 year and bi-monthly till the end of the 2nd postoperative year. Erythrocyte deformability was measured by bulk filtrometry (relative cell transit time, RCTT) and on the 20th-24th postoperative months by ektacytometry (elongation index, EI), too. RCTT was elevated in operated groups till the 12th month. On the 20th/24th months "SE"-group expressed the highest level, while the "AU"-groups were close to "Sham"-group. Using ektacytometer we found that on the 20th/24th month both EImax and SS1/2 showed the lowest values (because of unusual curve characteristic) in "SE"-group and "AU"-groups almost reached the "SH"-group. Spleen autotransplantation partially restored the normal rheological conditions. Both filtrometry and ektacytometry was suitable to investigate hyposplenic-asplenic conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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