Method for Quantitative Study of Airway Functional Microanatomy Using Micro-Optical Coherence Tomography
| Autor: | Guillermo J. Tearney, Marina Mazur, Yao Li, Suzanne Byan-Parker, Susan E. Birket, Gregory Dierksen, Eric J. Sorscher, Bradford Diephuis, Suresh Shastry, Linbo Liu, Kengyeh K. Chu, Eric J. Wilsterman, William E. Grizzle, Grace H. Houser, Steven M. Rowe |
|---|---|
| Přispěvatelé: | Harvard University--MIT Division of Health Sciences and Technology, Diephuis, Bradford J., Tearney, Guillermo J. |
| Rok vydání: | 2013 |
| Předmět: |
Respiratory Mucosa
Anatomy and Physiology Pulmonology Swine Respiratory System lcsh:Medicine Pediatrics 01 natural sciences Engineering Molecular Cell Biology lcsh:Science 0303 health sciences Multidisciplinary medicine.diagnostic_test Cilium Resolution (electron density) Anatomy medicine.anatomical_structure Mucociliary Clearance Medicine Cellular Types Tomography Optical Coherence Research Article Biotechnology Histology Mucociliary clearance Primary Cell Culture Biophysics Bioengineering Bronchi Biology 010309 optics 03 medical and health sciences Optical coherence tomography Diagnostic Medicine 0103 physical sciences medicine Animals Humans Respiratory Physiology Cilia 030304 developmental biology lcsh:R Epithelial Cells Mucus Epithelium lcsh:Q Airway Biomedical engineering |
| Zdroj: | PLoS ONE, Vol 8, Iss 1, p e54473 (2013) PLoS ONE PLoS |
| ISSN: | 1932-6203 |
| Popis: | We demonstrate the use of a high resolution form of optical coherence tomography, termed micro-OCT (μOCT), for investigating the functional microanatomy of airway epithelia. μOCT captures several key parameters governing the function of the airway surface (airway surface liquid depth, periciliary liquid depth, ciliary function including beat frequency, and mucociliary transport rate) from the same series of images and without exogenous particles or labels, enabling non-invasive study of dynamic phenomena. Additionally, the high resolution of μOCT reveals distinguishable phases of the ciliary stroke pattern and glandular extrusion. Images and functional measurements from primary human bronchial epithelial cell cultures and excised tissue are presented and compared with measurements using existing gold standard methods. Active secretion from mucus glands in tissue, a key parameter of epithelial function, was also observed and quantified. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|