The Combination of N-Acetyl Cysteine, Alpha-Lipoic Acid, and Bromelain Shows High Anti-Inflammatory Properties in NovelIn VivoandIn VitroModels of Endometriosis
| Autor: | Roberta Bulla, Chiara Agostinis, Giorgio Zauli, Sonia Zorzet, R. De Leo, F. De Seta |
|---|---|
| Přispěvatelé: | Agostinis, C., Zorzet, Sonia, De Leo, R., Zauli, G., DE SETA, Francesco, Bulla, Roberta |
| Rok vydání: | 2015 |
| Předmět: |
Pathology
Anti-Inflammatory Agents Apoptosis Mice SCID Pharmacology Acetylcysteine Mice and bromelain Endometriosi Cells Cultured Caspase Microscopy Cultured Thioctic Acid biology Bromelains endothelial cells N-acetyl cystein endothelial cell Female Tumor necrosis factor alpha Research Article lcsh:RB1-214 medicine.drug medicine.medical_specialty Article Subject medicine.drug_class Cells Immunology Endometriosis Vascular Cell Adhesion Molecule-1 SCID Fluorescence Anti-inflammatory NO Proinflammatory cytokine In vivo lcsh:Pathology medicine Animals Humans Animal Tumor Necrosis Factor-alpha Cell Biology inflammation alpha-lipoic acid and bromelain business.industry alpha-lipoic acid Uterus Disease Models Animal Endothelial Cells Inflammation Microscopy Fluorescence In vitro Disease Models biology.protein business |
| Zdroj: | Mediators of Inflammation, Vol 2015 (2015) Mediators of Inflammation |
| ISSN: | 1466-1861 0962-9351 |
| DOI: | 10.1155/2015/918089 |
| Popis: | To evaluate the efficacy of an association of N-acetyl cystein, alpha-lipoic acid, and bromelain (NAC/LA/Br) in the treatment of endometriosis we set up a newin vivomurine model. We explored the anti-inflammatory and proapoptotic effect of this combination on human endometriotic endothelial cells (EECs) and on endothelial cells isolated from normal uterus (UtMECs). We implanted fragments of human endometriotic cysts intraperitoneally into SCID mice to evaluate the efficacy of NAC/LA/Br treatment. UtMECs and EECs, untreated or treated with NAC/LA/Br, were activated with the proinflammatory stimulus TNF-αand their response in terms of VCAM1 expression was evaluated. The proapoptotic effect of higher doses of NAC/LA/Br on UtMECs and EECs was measured with a fluorogenic substrate for activated caspases 3 and 7. The preincubation of EECs with NAC/LA/Br prior to cell stimulation with TNF-αprevents the upregulation of the expression of the inflammatory “marker” VCAM1. Furthermore NAC/LA/Br were able to induce EEC, but not UtMEC, apoptosis. Finally, the novel mouse model allowed us to demonstrate that mice treated with NAC/LA/Br presented a lower number of cysts, smaller in size, compared to untreated mice. Our findings suggest that these dietary supplements may have potential therapeutic uses in the treatment of chronic inflammatory diseases like endometriosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|