Glycolytic and respiratory properties of intact mammalian cells: Inhibitor studies
| Autor: | C.T. Gregg, J.M. Machinist, W.D. Currie |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Oligomycin
Lymphoma Antimetabolites Manometry Cellular respiration Cell Biophysics Respiratory chain Antimycin A Uterine Cervical Neoplasms Biology Mitochondrion Biochemistry Phosphates Mice chemistry.chemical_compound Adenosine Triphosphate Oxygen Consumption Cricetinae Culture Techniques Rotenone medicine Animals Humans Glycolysis Glycosides Amino Acids Molecular Biology Carbon Isotopes Cyanides Chinese hamster ovary cell Ovary Phosphorus Isotopes Biological Transport Neoplasms Experimental In vitro Anti-Bacterial Agents Mitochondria Neoplasm Proteins medicine.anatomical_structure chemistry Depression Chemical Amobarbital Female Oligomycins HeLa Cells |
| Zdroj: | Archives of Biochemistry and Biophysics. 127:101-111 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/0003-9861(68)90206-3 |
| Popis: | The glycolytic and respiratory capacities of five cultured mammalian cell lines and a mouse ascites cell were compared. Mouse lymphoma cells (L5178Y) were studied in more detail. Inhibition of lymphoma cell respiration is linear and complete by rotenone, antimycin and Amytal, complete but nonlinear by cyanide, and linear but incomplete by oligomycin and aurovertin. The cells are substantially more sensitive to many of these inhibitors than are isolated rat liver mitochondria and fail to show the threshold phenomenon characteristic of mitochondria in vitro. These data show the feasibility of studying mitochondrial properties in vivo through the use of selective inhibitors. Of the agents tested, only atractyloside failed to inhibit respiration in intact cells. Studies of phosphate transport by Chinese hamster ovary cells and of valine incorporation by the lymphoma cells indicate that the energy requirements for these two processes are met in very different ways. Energy for the first process is supplied nearly equally by glycolytic and respiratory chain ATP; nonphosphorylated high-energy intermediates of the respiratory chain appear to play no part. Valine incorporation, in contrast, appears to be driven exclusively by respiratory chain energy with both ATP and nonphosphorylated high-energy intermediates contributing. |
| Databáze: | OpenAIRE |
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