Auxin and cytokinin control formation of the quiescent centre in the adventitious root apex of arabidopsis

Autor: S. D'Angeli, Laura Fattorini, F. Della Rovere, Maria Maddalena Altamura, Giuseppina Falasca, A Veloccia
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Cytokinins
Indoles
Arabidopsis thaliana
WOX5
quiescent centre
Arabidopsis
Adventitious root apex
Plant Science
Plant Roots
Hypocotyl
Mixed Function Oxygenases
chemistry.chemical_compound
Isopentenyladenosine
Gene Expression Regulation
Plant
root meristemoids
stem endodermis
heterocyclic compounds
Glucuronidase
chemistry.chemical_classification
food and beverages
Cell biology
Biochemistry
Cytokinin
cytokinin localization
Cell Division
Zeatin
Biology
Genes
Plant
Models
Biological
Auxin
adventitious root apex
thin cell layers
auxin transport
auxin biosynthesis
wox5
arabidopsis thaliana
Homeodomain Proteins
Indoleacetic Acids
Arabidopsis Proteins
fungi
Membrane Transport Proteins
Biological Transport
Original Articles
Kinetin
biology.organism_classification
Pericycle
chemistry
Seedlings
Endodermis
Biomarkers
Zdroj: Annals of Botany
ISSN: 1095-8290
0305-7364
Popis: †BackgroundandAimsAdventitiousroots(ARs)arepartoftherootsysteminnumerousplants,andarerequiredforsuccessful micropropagation. In the Arabidopsis thaliana primary root (PR) and lateral roots (LRs), the quiescentcentre(QC)inthestemcellnicheofthemeristemcontrolsapicalgrowthwiththeinvolvementofauxinandcytokinin.In arabidopsis, ARs emerge in planta from the hypocotyl pericycle, and from different tissues in in vitro culturedexplants, e.g. from the stem endodermis in thin cell layer (TCL) explants. The aim of this study was to investigatetheestablishmentandmaintenanceoftheQCinarabidopsisARs,inplantaandinTCLexplants,becauseinformationabout this process is still lacking, and it has potential use for biotechnological applications.†Methods Expression of PR/LR QC markers and auxin influx (LAX3)/efflux (PIN1) genes was investigated in thepresence/absenceofexogenousauxinandcytokinin.AuxinwasmonitoredbytheDR5::GUS systemandcytokininbyimmunolocalization.Theexpressionoftheauxin-biosyntheticYUCCA6genewasalsoinvestigatedbyinsituhy-bridizationinplantaandinAR-formingTCLsfromtheindoleaceticacid(IAA)-overproducingsuperroot2-1mutantand its wild type.†Key Results The accumulation of auxin and the expression of the QC marker WOX5 characterized the earlyderivatives of the AR founder cells, in planta and in in vitro cultured TCLs. By determination of PIN1 auxinefflux carrier and LAX3 auxin influx carrier activities, an auxin maximum was determined to occurat the AR tip,to which WOX5 expression was restricted, establishing the positioning of the QC. Cytokinin caused a restrictionof LAX3 and PIN1 expression domains, and concomitantly the auxin biosynthesis YUCCA6 gene was expressedin the apex.†Conclusions In ARs formed in planta and TCLs, the QC is established in a similar way, and auxin transport andbiosynthesisare involved through cytokinintuning.Key words: Adventitious root apex, Arabidopsis thaliana, auxin biosynthesis, auxin transport, cytokininlocalization, quiescentcentre, rootmeristemoids, stem endodermis, thin cell layers, WOX5.
Databáze: OpenAIRE
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