Reproductive seasonality and sperm cryopreservation in the male tufted deer (Elaphodus cephalophus)
| Autor: | Mongkol Techakumphu, Saritvich Panyaboriban, Dolores Reed, Nucharin Songsasen, Janine L. Brown, Ram Pratap Singh, Luis R. Padilla, Budhan S. Pukazhenthi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
Semen Biology Electroejaculation Cryopreservation Andrology 03 medical and health sciences 0302 clinical medicine Food Animals Reproductive biology Animals Small Animals Acrosome Sperm motility 030219 obstetrics & reproductive medicine urogenital system Equine Deer 0402 animal and dairy science 04 agricultural and veterinary sciences biology.organism_classification Spermatozoa 040201 dairy & animal science Sperm Animals Zoo Animal Science and Zoology Seasons Semen Preservation Tufted deer |
| Zdroj: | Theriogenology. 86:914-923 |
| ISSN: | 0093-691X |
| DOI: | 10.1016/j.theriogenology.2016.03.014 |
| Popis: | The tufted deer is a small deer, listed as near threatened on the International Union for Conservation of Nature Red List, and there is no information on the fundamental reproductive biology of this species. In this study, we report for the first time, characterization of male reproductive traits and cryopreservation of semen in this species. Males were subjected to electroejaculation during each season (autumn, winter, spring, and summer), and ejaculates were assessed for motility and quality traits. Fecal samples were collected 3 to 5 times weekly for 2 years and analyzed for androgen metabolites using enzyme immunoassay. Ejaculates with greater than 70% motility were cryopreserved using Beltsville extender (BF5F) or Triladyl. Straws were thawed and assessed subjectively as well as swim-up processed to isolate motile spermatozoa for computer-assisted sperm analysis and acrosome integrity at hourly interval. Tufted deer male reproductive and semen traits peaked in autumn. Mean fecal androgen concentrations were highest in the summer compared with baseline values during rest of the year. Sperm motility and acrosome integrity were lower immediately after thawing in both cryodiluents compared with raw ejaculates. Motility characteristics after swim-up were higher in BF5F compared with Triladyl. Four hours after thawing, both percent sperm motility and progression decreased further and were similar between BF5F and Triladyl. However, the proportion of spermatozoa with intact acrosomal membranes was higher in BF5F than Triladyl. Results indicate that tufted deer exhibit seasonal variations in reproductive traits and that BF5F better preserves sperm motility and acrosomal integrity after cryopreservation compared with Triladyl. |
| Databáze: | OpenAIRE |
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